SRF protein is upregulated during stretch-induced hypertrophy of rooster ALD muscle.
SRF protein is upregulated during stretch-induced hypertrophy of rooster ALD muscle.
复制标题
SRF 蛋白在拉伸引起的公鸡 ALD 肌肉肥大过程中上调。
DOI：
10.1152/jappl.1999.86.6.1793
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Booth,FW
中科院分区：
文献类型：
--
作者：
Fluck,M;Carson,JA;Schwartz,RJ;Booth,FW
血清反应因子1在公鸡背前阔肌肥大（ALD）期间是激活骨骼α-肌动蛋白启动子的必要和充分条件[J]。A.卡森，R. J.施瓦茨和F. W.布斯。[j].中国生物医学工程学报，2016,31(2):387 - 398。血清反应因子（SRF）蛋白是一种与血清反应元件1结合并激活骨骼α-肌动蛋白启动子的同源二聚体转录因子。外源SRF蛋白在复制的c2c12成肌细胞中的表达增加可诱导骨骼α-肌动蛋白启动子的三到四倍激活（魏丽丽，周文伟，J. D. Croissant, f . e .）。约翰森，R. Prywes， A. Balasubramamyan和R. J. schwartz。医学杂志。化学。273:30287-30294,1998)。因此，我们假设SRF蛋白浓度会在骨骼肌肥大期间升高。在本研究中，将公鸡体重的10%附着在左翅上，以诱导ALD肌肉比对侧肌肉增大。Western分析显示，在肥厚的第7天和第13天，ALD肌肉每克湿重的SRF蛋白显著增加。此外，在拉伸7天的ALD肌肉中，粗核蛋白和细胞质部分均出现SRF蛋白的增加。这是第一个显示转录因子蛋白浓度增加的报告，该转录因子在骨骼α-肌动蛋白启动子中的调节元件先前已被证明是动物过载骨骼肌肥厚信号转导所必需的。
Serum response element 1 has previously been reported to be necessary and sufficient for activation of the skeletal α-actin promoter during hypertrophy of the anterior latissimus dorsi (ALD) muscle of roosters [J. A. Carson, R. J. Schwartz, and F. W. Booth.Am. J. Physiol.270 (Cell Physiol.39): C1624–C1633, 1996]. Serum response factor (SRF) protein is the transcription factor that binds as a homodimer to serum response element 1 and activates the skeletal α-actin promoter. An increased expression of exogenous SRF protein in replicating C2C12myoblasts induced a three- to fourfold activation of the skeletal α-actin promoter (L. Wei, W. Zhou, J. D. Croissant, F.-E. Johansen, R. Prywes, A. Balasubramamyan, and R. J. Schwartz.J. Biol. Chem. 273: 30287–30294, 1998). Thus we hypothesized that SRF protein concentration would be increased during hypertrophy of skeletal muscle. In the present study, 10% of the rooster’s body weight was attached to the left wing to induce enlargement of the ALD muscle compared with the contralateral muscle. With Western analysis, a significant increase in SRF protein per gram of wet weight of the ALD muscle was noted at 7 and 13 days of hypertrophy. Furthermore, the increase in SRF protein occurred in both crude nuclear protein and cytoplasmic fractions in 7-day stretched ALD muscles. This is the first report showing increased protein concentration for a transcription factor whose regulatory element in the skeletal α-actin promoter has previously been shown to be required for the transduction of a hypertrophy signal in overloaded skeletal muscle of an animal.