Radioimmunoassay for anaphylatoxins: a sensitive method for determining complement activation products in biological fluids.
Radioimmunoassay for anaphylatoxins: a sensitive method for determining complement activation products in biological fluids.
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过敏毒素放射免疫测定：一种测定生物体液中补体激活产物的灵敏方法。
DOI：
10.1016/0003-2697(84)90308-7
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发表时间：
1984
影响因子：
2.9
通讯作者：
Hugli,TE
中科院分区：
文献类型：
--
作者：
Wagner,JL;Hugli,TE
血液补体系统的激活产生称为过敏毒素的生物活性片段。三种过敏毒素C3 a、C4 a和C5 a在“经典途径”激活期间释放，而当补体的“替代途径”激活时仅释放C3 a和C5 a。放射免疫测定被设计为单独检测和定量生物液体中的活化片段C3 a、C4 a和C5 a，而不受前体分子C3、C4和C5的干扰。采用放射免疫分析技术，对暴露于激活剂酵母聚糖、热聚集免疫球蛋白或眼镜蛇毒因子的新鲜人血清中补体激活的动力学进行了监测。首次在单一血清样品中同时进行了组分C3、C4和C5的活化。分析血清活化过程中过敏毒素形成的模式和程度可用于筛选补体级联反应的缺陷或缺陷。新鲜抽取的血清中过敏毒素的水平远高于EDTA-血浆中检测到的水平。血浆中过敏毒素的检测限分别由C3 a、C4 a和C5 a的背景水平152 ± 69、155 ± 33和5.4 ± 6.6 ng/ml控制。使用过敏毒素形成作为指标，检测患者血液、尿液或滑液中的低水平补体活化，可证明可用于发出多种形式的炎症反应的信号。临床样本中过敏毒素的证明被认为是监测免疫性疾病发作的有价值的诊断工具。
Activation of the blood complement system generates bioactive fragments called anaphylatoxins. The three anaphylatoxins C3a, C4a, and C5a are released during “classical pathway” activation while only C3a and C5a are released when the “alternative pathway” of complement is activated. Radioimmunoassays were designed to individually detect and quantitate the activation fragments C3a, C4a, and C5a in biological fluids without interference from the precursor molecules C3, C4, and C5. Kinetics of complement activation in fresh human serum exposed to the activators zymosan, heat-aggregated immunoglobulin, or cobra venom factor were monitored using the radioimmunoassay technique. For the first time, activation of components C3, C4, and C5 was followed simultaneously in a single serum sample. Analysis of the patterns and extent of anaphylatoxin formation during activation in serum may be used to screen for deficiencies or defects in the complement cascade. Levels of the anaphylatoxins in freshly drawn serum were much higher than levels detected in EDTA-plasma. Detection limits of anaphylatoxins in plasma are governed by background levels of 152 ± 69, 155 ± 33, and 5.4 ± 6.6 ng/ml for C3a, C4a, and C5a, respectively. Detection of low-level complement activation in patient's blood, urine, or synovial fluid, using anaphylatoxin formation as an indicator, may prove useful in signaling numerous forms of inflammatory reactions. The demonstration of anaphylatoxins in clinical samples is being recognized as a valuable diagnostic tool in monitoring the onset of immune disease.