SINGLE-CELL ANALYSIS OF CYTOKINE GENE COEXPRESSION DURING CD4(+) T-CELL PHENOTYPE DEVELOPMENT
SINGLE-CELL ANALYSIS OF CYTOKINE GENE COEXPRESSION DURING CD4(+) T-CELL PHENOTYPE DEVELOPMENT
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DOI：
10.1073/pnas.92.16.7565
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发表时间：
1995-08-01
影响因子：
11.1
通讯作者：
WEAVER, CT
中科院分区：
文献类型：
--
作者：
BUCY, RP;KARR, L;WEAVER, CT
采用双标记原位杂交技术分析了α β-T细胞受体转基因小鼠的CD4（+）T细胞在表型发育过程中细胞因子mRNA的共表达。在初次应答中产生细胞因子的T细胞是活化群体的一部分，并且只有少数的精氨酸阳性细胞共表达两种细胞因子。在二次反应中，双阳性细胞的频率增加，尽管它们仍然是总数的少数。在检查的细胞因子对中，白细胞介素（IL）-4和IL-5是最常共表达的。IL-4和干扰素γ表现出最大的倾向，分离的表达，很少共表达后的主要刺激。这些数据表明，在早期抗原应答过程中，单个CD4（+）T细胞的细胞因子基因表达存在显著的异质性。任何细胞因子对的共表达，更不用说Th1和Th2细胞因子，通常是例外。Th0表型是群体表型而不是单个细胞表型。
CD4(+) T cells from alpha beta-T-cell receptor transgenic mice were analyzed for coexpression of cytokine mRNAs during phenotype development using a double-label in situ hybridization technique. T cells that produced cytokines in the primary response were a fraction of the activated population, and only a minority of the cytokine-positive cells coexpressed two cytokines. In secondary responses, frequencies of double-positive cells increased, although they remained a minority of the total. Of the cytokine pairs examined, interleukin (IL)-4 and IL-5 were the most frequently coexpressed. IL-4 and interferon gamma showed the greatest tendency toward segregation of expression, being rarely coexpressed after the primary stimulation. These data indicate that there is significant heterogeneity of cytokine gene expression by individual CD4(+) T cells during early antigenic responses. Coexpression of any pairs of cytokines, much less Th1 and Th2 cytokines, is generally the exception. The Th0 phenotype is a population phenotype rather than an individual cell phenotype.