Conjugation of magnetic beads for immunopurification of protein complexes.
Conjugation of magnetic beads for immunopurification of protein complexes.
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DOI：
10.1101/pdb.prot5611
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发表时间：
2011-05-01
影响因子：
--
通讯作者：
Chait, Brian T
中科院分区：
文献类型：
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作者：
Cristea, Ileana M;Chait, Brian T
介绍：蛋白质复合物的组成知识提供了对其功能的关键见解。免疫亲和纯化为分离蛋白质复合物和阐明其组成提供了有效的手段。免疫分离是用针对感兴趣的蛋白质或与感兴趣的蛋白质偶联的标签的抗体来实现的。这种方法使用免疫亲和纯化的磁珠包被抗体快速和有效地纯化蛋白质复合物从细胞或组织。本协议描述了使用共轭磁珠亲和纯化蛋白质复合物。介绍：蛋白质复合物的组成知识提供了对其功能的关键见解。免疫亲和纯化为分离蛋白质复合物和阐明其组成提供了有效的手段。免疫分离是用针对感兴趣的蛋白质或与感兴趣的蛋白质偶联的标签的抗体来实现的。这种方法使用免疫亲和纯化的磁珠包被抗体快速和有效地纯化蛋白质复合物从细胞或组织。本方案描述了磁珠与抗体的偶联，以制备蛋白质复合物的免疫沉淀。
INTRODUCTION: Knowledge of the composition of protein complexes provides key insights into their functions. Immunoaffinity purification provides an effective means for isolating protein complexes and elucidating their composition. Immunoisolation is achieved with antibodies directed either specifically against the proteins of interest or against tags that are coupled to the proteins of interest. This approach uses immunoaffinity purification on magnetic beads coated with antibodies for the rapid and efficient purification of protein complexes from cells or tissues. This protocol describes affinity purification of protein complexes using conjugated magnetic beads.INTRODUCTION: Knowledge of the composition of protein complexes provides key insights into their functions. Immunoaffinity purification provides an effective means for isolating protein complexes and elucidating their composition. Immunoisolation is achieved with antibodies directed either specifically against the proteins of interest or against tags that are coupled to the proteins of interest. This approach uses immunoaffinity purification on magnetic beads coated with antibodies for the rapid and efficient purification of protein complexes from cells or tissues. This protocol describes conjugation of magnetic beads with antibodies in preparation for immunopreciptation of protein complexes.