Relocation of NPM Affects the Malignant Phenotypes of Hepatoma SMMC-7721 Cells
Relocation of NPM Affects the Malignant Phenotypes of Hepatoma SMMC-7721 Cells
复制标题
NPM 的重新定位影响肝癌 SMMC-7721 细胞的恶性表型。
DOI：
10.1002/jcb.25971
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发表时间：
2017
影响因子：
4
通讯作者：
Shi Song-Lin
中科院分区：
文献类型：
--
作者：
Li Xiao;Xu Dong-Hui;Liu Fan;Liu Guo-Yan;Lu Kun;Deng Xiao-Ling;Li Qi-Fu;Shi Song-Lin
Nucleophosmin(NPM), heavily implicated in diverse solid tumors, is an important multifunctional protein mainly located in the nucleolus. Our previous study confirmed that NPM can also localize and accumulate in the cytoplasm of liver cancer cells. However, the role of cytoplasmic NPM (NPMc +) is unclear. Here, we showed that both nucleolar NPM and NPMc+ could promote cell proliferation, although the effect of NPMc+ was weaker than that of NPM. Cell adhesion ability of hepatoma cells was significantly reduced to a greater extent by NPMc+ expression. Nucleolar NPM enhanced cell migration and invasion, whereas NPMc+ impeded cell migration and invasion. The investigation of NPM interactional proteins by proteomic method demonstrated that the NPM was involved in multiple biological processes. By contrast, the interactional proteins of NPMc+ were mainly implicated in tRNA amino acylation regulation. The interactional network of NPMc+ was significantly small and simple. These results suggested that relocation of NPM altered its interactional network and consequently disturbed the primary functions, including cell proliferation, adhesion, migration, and invasion. NPM plays a promotional role in cancer and the reducing relocation may be a potential therapeutic target for hepatocellular carcinoma. J. Cell. Biochem. 118: 3225–3236, 2017. © 2017 Wiley Periodicals, Inc.
