STREPTOMYCIN INDUCED RELEASE OF FMET-TRANSFER RNA FROM RIBOSOMAL INITIATION COMPLEX
STREPTOMYCIN INDUCED RELEASE OF FMET-TRANSFER RNA FROM RIBOSOMAL INITIATION COMPLEX
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DOI：
10.1016/0006-291x(71)90403-7
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发表时间：
1971-01-01
影响因子：
3.1
通讯作者：
GROS, F
中科院分区：
文献类型：
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作者：
LELONG, JC;COUSIN, MA;GROS, F
研究了链霉素对体外mRNA翻译起始步骤的影响。该药物不抑制起始tRNA位于30 S-核糖体-mRNA复合物上的初级组装步骤，但导致结合的fMet-tRNA从预先形成的起始复合物中释放。所观察到的两个亚基和GTP水解的要求表明，fMet-tRNA不能被释放，直到结合到敏感的核糖体上的嘌呤霉素反应位点。其他引起误读的氨基糖苷类抗生素不诱导fMet-tRNA释放，表明链霉素特异性诱导P位点的扭曲。
The streptomycin effect on the initial steps of mRNA translation in vitro was studied. The drug does not inhibit the primary assembling step during which initiator tRNA is positioned on a 30S-ribosome-mRNA complex but causes release of bound fMet-tRNA from the preformed initiation complex. The observed requirement for both subunits and GTP hydrolysis indicated that fMet-tRNA could not be released until bound to the puromycin reactive site on sensitive ribosomes. Other aminoglycoside antibiotics which cause misreading do not induce fMet-tRNA release showing that streptomycin specifically induces distortion of the P site.