The calcium-binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half bridge of the spindle pole body
The calcium-binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half bridge of the spindle pole body
复制标题
酿酒酵母钙结合蛋白细胞分裂周期31是纺锤体极体半桥的组成部分
作者：
A. Spang;I. Courtney;Ursel Fackler;M. Matzner;E. Schiebel
酿酒酵母的Cdc 31突变体在非允许温度下停滞，具有大芽、G2 DNA含量和单个异常大的纺锤体极体（SP B）（Byers，B. 1981.酵母分子遗传学阿尔弗雷德·本松研讨会。16：119-133）。在这份报告中，我们表明，CDC 31基因的产品是必不可少的细胞活力。我们证明，纯化的CDC 31蛋白结合Ca 2+，这种结合是高度特异性的。综上所述，三条证据表明CDC 31是SPB的一个组分。首先，CDC 31与SPB的富集制剂共分馏。其次，免疫荧光染色表明，CDC 31与已知的SPB组分共定位。第三，免疫电子显微镜与全细胞和与分离的SPB显示，CDC 31是本地化的SPB的半桥，它位于紧邻SPB斑块。CDC 31主要在半桥的细胞质侧检测到，因此，定义了SPB的进一步亚结构。我们认为，CDC 31是一个家庭的成员，钙结合，中心体相关的蛋白质从一个遗传多样性组的生物体。
Cdc31 mutants of Saccharomyces cerevisiae arrest at the nonpermissive temperature with large buds, G2 DNA content and, a single, abnormally large spindle pole body (SPB) (Byers, B. 1981. Molecular Genetics in Yeast. Alfred Benzon Symposium. 16:119-133). In this report, we show that the CDC31 gene product is essential for cell viability. We demonstrate that purified CDC31 protein binds Ca2+ and that this binding is highly specific. Taken together, three lines of evidence indicate that CDC31 is a component of the SPB. First, CDC31 cofractionates with enriched preparations of SPBs. Second, immunofluorescence staining indicates that CDC31 colocalizes with a known SPB component. Third, immunoelectron microscopy with whole cells and with isolated SPBs reveals that CDC31 is localized to the half bridge of the SPB, which lies immediately adjacent to the SPB plaques. CDC31 was detected mainly at the cytoplasmic side of the half bridge and, therefore, defines a further substructure of the SPB. We suggest that CDC31 is a member of a family of calcium-binding, centrosome- associated proteins from a phylogenetically diverse group of organisms.
DOI：
10.1073/pnas.82.4.1074
发表时间：
1985-01-01
影响因子：
11.1
作者：
TABOR, S;RICHARDSON, CC
通讯作者：
RICHARDSON, CC
DOI：
10.1101/sqb.1991.056.01.075
发表时间：
1991
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
R. Sikorski;W. Michaud;J. Wootton;M. Boguski;C. Connelly;P. Hieter
通讯作者：
R. Sikorski;W. Michaud;J. Wootton;M. Boguski;C. Connelly;P. Hieter
影响因子：
3.3
作者：
ANTEBI, A;FINK, GR
通讯作者：
FINK, GR