Dissolved organic phosphorus utilization by the marine bacterium Ruegeria pomeroyi DSS-3 reveals chain length-dependent polyphosphate degradation.
Dissolved organic phosphorus utilization by the marine bacterium Ruegeria pomeroyi DSS-3 reveals chain length-dependent polyphosphate degradation.
复制标题
DOI：
10.1111/1462-2920.15877
复制
发表时间：
2022-05
影响因子：
5.1
通讯作者：
Diaz, Julia M.
中科院分区：
文献类型：
--
作者：
Adams, Jamee C.;Steffen, Rachel;Chou, Chau-Wen;Duhamel, Solange;Diaz, Julia M.
溶解有机磷（DOP）是海洋微生物群落的重要营养资源。然而，人们对不同类型 DOP（例如磷酸单酯 (P-O-C) 和磷酸酐 (P-O-P)）的相对生物利用度知之甚少。在这里，我们评估了代表性的富营养细菌 Ruegeria pomeroyi DSS-3 对这些磷源的利用。所有 DOP 来源都支持 R. pomeroyi 的同等生长，并且所有 DOP 水解率在磷消耗 (-P) 下均上调。长链聚磷酸盐 (45polyP) 在所有测试的 DOP 底物中表现出最低的水解率，包括三聚磷酸盐 (3polyP)。然而，-P 条件下 45polyP 水解的上调大于任何其他分析底物。蛋白质组学揭示了三种常见的磷获取酶可能参与多磷酸盐的利用，包括两种碱性磷酸酶，PhoD 和 PhoX，以及一种 5'-核苷酸酶 (5'-NT)。 DOP 底物竞争实验的结果表明，这些酶可能具有广泛的底物特异性，包括对多磷酸盐的链长度依赖性反应性。这些结果证实，DOP（包括聚磷）是波默罗氏菌以及可能的其他海洋异养细菌的生物可利用的营养磷来源。此外，polyP 水解的链长依赖性机制、速率和调节表明，这些过程可能会影响 DOP 的组成以及海洋溶解有机物中养分的整体回收。
Dissolved organic phosphorus (DOP) is a critical nutritional resource for marine microbial communities. However, the relative bioavailability of different types of DOP, such as phosphomonoesters (P‐O‐C) and phosphoanhydrides (P‐O‐P), is poorly understood. Here we assess the utilization of these P sources by a representative bacterial copiotroph, Ruegeria pomeroyi DSS‐3. All DOP sources supported equivalent growth by R. pomeroyi, and all DOP hydrolysis rates were upregulated under phosphorus depletion (−P). A long‐chain polyphosphate (45polyP) showed the lowest hydrolysis rate of all DOP substrates tested, including tripolyphosphate (3polyP). Yet the upregulation of 45polyP hydrolysis under −P was greater than any other substrate analyzed. Proteomics revealed three common P acquisition enzymes potentially involved in polyphosphate utilization, including two alkaline phosphatases, PhoD and PhoX, and one 5′‐nucleotidase (5′‐NT). Results from DOP substrate competition experiments show that these enzymes likely have broad substrate specificities, including chain length‐dependent reactivity toward polyphosphate. These results confirm that DOP, including polyP, are bioavailable nutritional P sources for R. pomeroyi, and possibly other marine heterotrophic bacteria. Furthermore, the chain‐length dependent mechanisms, rates and regulation of polyP hydrolysis suggest that these processes may influence the composition of DOP and the overall recycling of nutrients within marine dissolved organic matter.
登录
查看更多内容
影响因子：
11.4
作者：
Diaz, Julia M.;Ingall, Ellery D.
通讯作者：
Ingall, Ellery D.
影响因子：
3.7
作者：
Cui, Yudong;Lin, Xin;Lin, Senjie
通讯作者：
Lin, Senjie
影响因子：
4.5
作者：
Martin, Patrick;Lauro, Federico M.;Vinayachandran, P. N.
通讯作者：
Vinayachandran, P. N.
影响因子：
56.9
作者：
Diaz, Julia;Ingall, Ellery;Brandes, Jay A.
通讯作者：
Brandes, Jay A.
影响因子：
5.1
作者：
Diaz, Julia M.;Steffen, Rachel;Duhamel, Solange
通讯作者：
Duhamel, Solange