Cryopreservation of cord blood after liquid storage
Cryopreservation of cord blood after liquid storage
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DOI：
10.1080/14653240310003035
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发表时间：
2003-01-01
期刊：
影响因子：
4.5
通讯作者：
McCullough, J
中科院分区：
文献类型：
--
作者：
Hubel, A;Carlquist, D;McCullough, J
背景：脐带血的处理可能导致红细胞消耗和冷冻保存之前的延迟。本研究的总体目的是确定冷冻前液体储存对解冻后存活能力的影响。方法在知情同意的情况下，从正常献血者采血补充CPD抗凝剂（CB）。将CB未稀释或以1:1比例的STM-sav储存72 h。未稀释的对照样品在室温下储存。添加STM-sav的CB样品在4℃保存。储存方案完成后，样品被RBC耗尽冷冻，储存，解冻，并测定其活力。有核细胞计数、CD34（+）细胞百分比和集落形成频率在液体储存期间和冷冻保存后进行测定。结果脐带血解冻后保存72 h的单核细胞恢复率和活力明显低于冷冻前保存24 h的脐带血。对于储存在STM-sav和对照组中的脐带血，这种差异是真实的。用STM-sav扩张脐带血可提高CFU-GM的发生率。低温保存前脐带血液体储存24小时不会对解冻后细胞恢复产生不利影响。存储解决方案（STM-Sav）的使用增强了解冻后菌落形成能力的保留。这些和其他研究为制定脐带血银行综合方案提供了重要基础。
BackgroundThe processing of cord blood may result in delays prior to RBC depletion and cryopreservation. The overall objective of this investigation is to determine the influence of liquid storage prior to cryopreservation on the post-thaw viability.MethodsUC blood supplemented with CPD anticoagulant (CB) was obtained from normal donors with informed consent. CB was stored undiluted or diluted with 1:1 ratio of storage solution STM-sav for up to 72 h. The undiluted control samples were stored at room temperature. CB samples supplemented with STM-sav were stored at 4degreesC. After completion of the storage protocol, the sample was RBC depleted frozen, stored, thawed, and assayed fir viability. Nucleated cell counts, percentage of CD34(+) cells, and frequency of colony formation were determined during liquid storage and after cryopreservation.ResultsThe post-thaw mononuclear cell recovery and viability (if cord blood stored for 72 h was significantly lower than that of cord blood stored for 24 h prior to cryopreservation. This difference was true fir cord bloods stored in STM-sav and controls. Dilation of the cord blood with STM-sav improved the frequency of CFU-GM observed.DiscussionLiquid storage of cord blood for 24 h prior to cryopreservation does not adversely influence the post-thaw cell recovery. The use of a storage solution (STM-Sav) enhances the retention of colony-forming capabilities post-thaw. These and other studies provide an important foundation for the development of integrated protocols for cord blood banking.