Investigation of age-specific behavioral and proteomic changes in an animal model of chronic ethanol exposure.
Investigation of age-specific behavioral and proteomic changes in an animal model of chronic ethanol exposure.
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慢性乙醇暴露动物模型中年龄特异性行为和蛋白质组变化的研究。
DOI：
10.1007/978-1-61779-458-2_30
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Kirstein,CherylL
中科院分区：
文献类型：
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作者：
Maldonado-Devincci,AntonietteM;StevensJr,StanleyM;Kirstein,CherylL
青春期饮酒是一个重大的健康问题，因为这是大脑继续经历关键发育变化的时期。许多行为研究已经在酒精暴露的动物模型中进行，并且已经确定了青春期的脆弱时期，这表明青春期酒精暴露的持久影响。然而，识别作为青春期暴露于乙醇的结果观察到的行为结果的分子变化仍然是一个重大的技术挑战。在这一章中，我们描述了一种方法，允许通过全球范围的蛋白质表达分析，以及在青少年和成年大鼠的行为反应性的评价，在青春期相对于成年期的慢性乙醇暴露的影响进行评估。从这种类型的分析结果可以促进识别与行为变化相关的年龄特异性分子标记物与乙醇治疗后，或在其他动物模型的药物滥用。
Alcohol use during adolescence represents a major health concern given that this is a period in which the brain continues to undergo critical developmental changes. Much behavioral research has been conducted in animal models of alcohol exposure, and a vulnerable period in adolescence has been identified that suggests lasting effects of ethanol exposure during adolescence. However, identification of molecular changes underlying the behavioral outcomes observed as a result from exposure to ethanol during adolescence remains a major technical challenge. In this chapter, we describe a method that allows for assessment of the effects of chronic ethanol exposure during adolescence relative to adulthood through global-scale analysis of protein expression as well as evaluation of behavioral responsivity in adolescent and adult rats. Results from this type of analysis can facilitate identification of age-specific molecular markers associated with behavioral changes following treatment with ethanol or in other animal models of drug abuse.