THE P50 SUBUNIT OF NF-KAPPA-B ASSOCIATES WITH THE NF-IL6 TRANSCRIPTION FACTOR
THE P50 SUBUNIT OF NF-KAPPA-B ASSOCIATES WITH THE NF-IL6 TRANSCRIPTION FACTOR
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DOI：
10.1073/pnas.89.17.8145
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发表时间：
1992-09-01
影响因子：
11.1
通讯作者：
SHARP, PA
中科院分区：
文献类型：
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作者：
LECLAIR, KP;BLANAR, MA;SHARP, PA
核因子-kappa-B-p50多肽是Rel转录因子家族的成员之一，是一种融合蛋白，含有氨基末端多肽，便于纯化和用单抗进行检测，并通过体外磷酸化进行特异性放射性标记。用P-32标记的NF-kappa-B-p50融合多肽作为探针进行Western blotting实验和噬菌体表达文库的筛选，以分离编码相互作用蛋白结构域的cDNA。正如预期的那样，编码REL家族蛋白的cDNA被鉴定出来。令人惊讶的是，P-32标记的NF-kappa-B蛋白也特异性地结合到由人NF-IL6转录因子的cDNA编码的蛋白质上。NF-kappa-B-p50和NF-IL6蛋白直接相互作用，而Rel同源结构域和亮氨酸拉链基序分别对这种相互作用起重要作用。由于核因子-kappaB和核因子-IL6的诱导是免疫和急性期反应中的重要事件，这种相互作用可能允许基因的共同调节。
The NF-kappa-B-p50 polypeptide, a member of the Rel family of transcription factors, was produced as a fusion protein containing amino-terminal peptide additions that facilitate purification and detection with a monoclonal antibody and specific radiolabeling by phosphorylation in vitro. The P-32-labeled NF-kappa-B-p50 fusion polypeptide was used as the probe in Western blotting experiments and in screenings of a bacteriophage expression library to isolate cDNAs encoding interacting protein domains. As expected, cDNAs encoding proteins of the Rel family were identified. Surprisingly, the P-32-labeled NF-kappa-B protein also specifically bound to proteins encoded by cDNAs for the human NF-IL6 transcription factor. The NF-kappa-B-p50 and NF-IL6 proteins directly interact, and the Rel homology domain and leucine-zipper motif, respectively, are important for this interaction. Since induction of the NF-kappa-B and NF-IL6 factors are important events in immune and acute-phase responses, this interaction could permit coregulation of genes.