CNN3 is regulated by microRNA-1 during muscle development in pigs.
CNN3 is regulated by microRNA-1 during muscle development in pigs.
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DOI：
10.7150/ijbs.8015
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发表时间：
2014
影响因子：
9.2
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Tang Z;Liang R;Zhao S;Wang R;Huang R;Li K
钙调蛋白3（CNN 3）基因在骨骼肌发育中具有重要的功能。microRNAs（miRNAs）通过影响靶基因mRNA的稳定性或蛋白质的翻译，在肌肉发生中发挥重要作用。基于对猪产前骨骼肌中microRNA和mRNA的配对分析，我们先前的研究表明CNN 3是差异表达的，并且是miR-1的潜在靶标。为了进一步了解CNN 3的生物学功能和调控机制，我们分别对通城猪（中国家养品种，肥胖型）和长白猪（西方品种，瘦肉型）骨骼肌组织发育（16个阶段）中CNN 3和miR-1的共表达进行了分析。随后，进行双荧光素酶和蛋白质印迹测定。在骨骼肌发育过程中，我们观察到miR-1和CNN 3在mRNA水平的表达呈显著负相关。我们的双荧光素酶和蛋白质印迹结果表明CNN 3基因受miR-1调控。我们确定了CNN 3基因内的四个单核苷酸多态性（SNP）。关联分析表明，这些CNN 3 SNPs与所检测仔猪的出生重（BW）和21天断奶重显著相关。这些事实表明CNN 3是与生长性状相关的候选基因，并且在猪骨骼肌发育过程中受到miR-1的调控。
The calponin 3 (CNN3) gene has important functions involved in skeletal muscle development. MicroRNAs (miRNAs) play critical role in myogenesis by influencing the mRNA stability or protein translation of target gene. Based on paired microRNA and mRNA profiling in the prenatal skeletal muscle of pigs, our previous study suggested that CNN3 was differentially expressed and a potential target for miR-1. To further understand the biological function and regulation mechanism of CNN3, we performed co-expression analysis of CNN3 and miR-1 in developmental skeletal muscle tissues (16 stages) from Tongcheng (a Chinese domestic breed, obese-type) and Landrace (a Western, lean-type) pigs, respectively. Subsequently, dual luciferase and western blot assays were carried out. During skeletal muscle development, we observe a significantly negative expression correlation between the miR-1 and CNN3 at mRNA level. Our dual luciferase and western blot results suggested that the CNN3 gene was regulated by miR-1. We identified four single nucleotide polymorphisms (SNPs) contained within the CNN3 gene. Association analysis indicated that these CNN3 SNPs are significantly associated with birth weight (BW) and the 21-day weaning weight of the piglets examined. These facts indicate that CNN3 is a candidate gene associated with growth traits and regulated by miR-1 during skeletal muscle development in pigs.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
DOI：
10.1083/jcb.200603008
发表时间：
2006-08-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kim HK;Lee YS;Sivaprasad U;Malhotra A;Dutta A
通讯作者：
Dutta A
影响因子：
3.3
作者：
Takaya, Tomohide;Ono, Koh;Hasegawa, Koji
通讯作者：
Hasegawa, Koji
影响因子：
3.3
作者：
McCarthy, John J.;Esser, Karyn A.
通讯作者：
Esser, Karyn A.
影响因子：
30.8
作者：
Chen, JF;Mandel, EM;Wang, DZ
通讯作者：
Wang, DZ