Phonophoretic delivery of 10% hydrocortisone through the epidermis of humans as determined by serum cortisol concentrations
Phonophoretic delivery of 10% hydrocortisone through the epidermis of humans as determined by serum cortisol concentrations
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DOI：
10.1093/ptj/76.7.738
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发表时间：
1996-07-01
期刊：
影响因子：
3.2
通讯作者：
Seal, LA
中科院分区：
文献类型：
--
作者：
Bare, AC;McAnaw, MB;Seal, LA
背景和目的。本研究的目的是确定是否应用氢化可的松超声波导入增强局部应用的氢化可的松在人类经皮输送，确定血液皮质醇水平。科目受试者为16名男性和女性，年龄在18岁至33岁之间（（X）超过标准线= 25，SD = 2.74），没有任何持续炎症性疾病的症状。方法.使用含有10%醋酸氢化可的松的凝胶偶联介质。在50 cm（2）区域内以1.0 W/cm（2）的强度和1.0 MHz的频率输送超声5分钟。每名受试者接受对照治疗（单独超声）和实验治疗（氢化可的松超声波）的前臂掌侧方面相隔1周。在对照和实验条件下，在每次给药前和给药后0、5和15分钟，从给药部位近端的肘静脉采血。采用荧光偏振免疫法测定血清皮质醇浓度。结果氢化可的松超声波电泳后血清皮质醇浓度没有升高。结论和讨论。这些发现表明，氢化可的松没有穿透表皮并进入下层血管。关于皮下组织内氢化可的松水平的临床意义进行了讨论，并建议进一步研究。
Background and Purpose. The purpose of this investigation was to determine whether application of hydrocortisone phonophoresis enhances transcutaneous delivery of topically applied hydrocortisone in humans, as determined by blood cortisol levels. Subjects. The subjects were 16 men and women, between the ages of 18 and 33 years ((X) over bar = 25, SD = 2.74), without symptoms of any ongoing inflammatory condition. Methods. A gel coupling medium containing 10% hydrocortisone acetate was used. Ultrasound was delivered over a 50-cm(2) area for 5 minutes at an intensity of 1.0 W/cm(2) and a frequency of 1.0 MHz. Each subjects received a control treatment (ultrasound alone) and an experimental treatment (hydrocortisone phonophoresis) on the volar aspect of the forearm 1 week apart. Blood was drawn, under both control and experimental conditions, from a cubital vein just proximal to the treatment site prior to each treatment and 0, 5, and 15 minutes posttreatment. Serum cortisol concentrations were measured using a fluorescence polarization immunoassay. Results. No rise in serum cortisol concentrations following hydrocortisone phonophoresis was detected. Conclusion and Discussion. These findings suggest that there was no penetration of hydrocortisone through the epidermis and into the underlying vasculature. Clinical implications regarding hydrocortisone levels within the subcutaneous tissues are discussed, and further research is suggested.