Alternative splicing and mRNA expression analysis of bovine SLAMF7 gene in healthy and mastitis mammary tissues
Alternative splicing and mRNA expression analysis of bovine SLAMF7 gene in healthy and mastitis mammary tissues
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牛SLAMF7基因在健康和乳腺炎乳腺组织中的选择性剪接和mRNA表达分析
DOI：
10.1007/s11033-011-1198-z
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发表时间：
2012-04-01
影响因子：
2.8
通讯作者：
Zhong, Jifeng
中科院分区：
文献类型：
--
作者：
Ju, Zhihua;Wang, Changfa;Zhong, Jifeng
信号传导淋巴细胞活化分子家族7（SLAMF 7）蛋白充当多种成熟造血细胞表面上的粘附分子，并且还部分控制某些先天性和适应性免疫应答。我们表征了三种新的牛SLAMF 7剪接变体，命名为SLAMF 7-AS 1、AS 2和AS 3。所有三种新的SLAMF 7同种型都是通过选择性剪接（AS）从完整转录物（SLAMF 7-完整）衍生的。三种剪接变体的模式是外显子跳跃和选择性5'剪接位点。牛SLAMF 7转录本在乳腺组织中表达，如通过实时PCR所证明的。正常乳腺组织中完整转录本的表达水平高于金黄色葡萄球菌（Staph.金黄色葡萄球菌）诱导的乳腺炎乳腺组织。但两种乳腺癌组织中mRNA表达水平比较差异无统计学意义。在正常和感染的乳腺组织中，SLAMF-AS 2亚型在三种转录物中表达水平最低。本研究为进一步了解牛SLAMF 7基因的功能提供了线索。
The signaling lymphocyte-activating molecule family 7 (SLAMF7) proteins serve as adhesion molecules on the surface of a variety of mature hematopoietic cells, and also partially control certain innate and adaptive immune responses. We characterized three novel bovine SLAMF7 splice variants, designated as SLAMF7-AS1, AS2, and AS3. All three novel SLAMF7 isoforms are derived from the complete transcripts (SLAMF7-complete) via alternative splicing (AS). The patterns of the three splice variants are exon skipping and alternative 5' splice sites. Bovine SLAMF7 transcripts are expressed in mammary tissue, as demonstrated by real-time PCR. The levels of the complete transcript expression in the normal mammary tissues were higher than that in Staphylococcus aureus (Staph. aureus)-induced mastitis mammary tissues. However, it was not significant for the mRNA expression level comparison between these two kinds of mammary. The SLAMF-AS2 isoforms are expressed the lowest levels among the three transcripts in both normal and infected mammary tissues. This study provides clues for a better understanding of bovine SLAMF7 gene function.