Topologically Micropatterned Collagen and Poly(ε-caprolactone) Struts Fabricated Using the Poly(vinyl alcohol) Fibrillation/Leaching Process To Develop Efficiently Engineered Skeletal Muscle Tissue
Topologically Micropatterned Collagen and Poly(ε-caprolactone) Struts Fabricated Using the Poly(vinyl alcohol) Fibrillation/Leaching Process To Develop Efficiently Engineered Skeletal Muscle Tissue
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DOI：
10.1021/acsami.7b14192
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发表时间：
2017-12-20
影响因子：
9.5
通讯作者：
Kim, GeunHyung
中科院分区：
文献类型：
--
作者：
Kim, Minseong;Kim, WonJin;Kim, GeunHyung
优化设计的三维（3D）生物医学支架骨骼肌组织再生提出了重大的研究挑战。目前，大多数关于支架的研究集中在二维（2D）表面结构上，这些结构在微米/纳米尺度上具有各种重复的尺寸和形状，以诱导成肌细胞的排列和肌管的形成。2D图案化表面清楚地提供了成肌细胞排列和分化的图案大小和形状的有效分析结果。然而，由于有限的厚度和3D形状，就直接应用于临床使用而言是不方便的。因此，本研究提出了一种创新的水凝胶或合成结构，其由单轴表面图案化的圆柱形支柱组成，用于骨骼肌再生。水凝胶（胶原）和聚（ε-己内酯）支柱上的图案的对齐是通过聚（乙烯醇）的原纤化和浸出过程来实现的。各种细胞培养结果表明，微图案化胶原结构上的C2 C12细胞完全对齐，并且当与未用微图案化过程处理的胶原结构相比时，实现了显著高水平的肌管形成。
Optimally designed three-dimensional (3D) biomedical scaffolds for skeletal muscle tissue regeneration pose significant research challenges. Currently, most studies on scaffolds focus on the two-dimensional (2D) surface structures that are patterned in the micro-/nanoscales with various repeating sizes and shapes to induce the alignment of myoblasts and myotube formation. The 2D patterned surface clearly provides effective analytical results of pattern size and shape of the myoblast alignment and differentiation. However, it is inconvenient in terms of the direct application for clinical usage due to the limited thickness and 3D shapeability. Hence, the present study suggests an innovative hydrogel or synthetic structure that consists of uniaxially surface-patterned cylindrical struts for skeleton muscle regeneration. The alignment of the pattern on the hydrogel (collagen) and poly(epsilon-caprolactone) struts was attained with the fibrillation of poly(vinyl alcohol) and the leaching process. Various cell culture results indicate that the C2C12 cells on the micropatterned collagen structure were fully aligned, and that a significantly high level of myotube formation was achieved when compared to the collagen structures that were not treated with the micropatterning process.