Centipeda periodontii gen. nov., sp. nov. from Human Periodontal Lesions
Centipeda periodontii gen. nov., sp. nov. from Human Periodontal Lesions
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牙周蜈蚣属
DOI：
10.1099/00207713-33-3-628
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发表时间：
1983
影响因子：
2.8
通讯作者：
M. Listgarten
中科院分区：
文献类型：
--
作者：
C. Lai;B. Males;P. Dougherty;P. Berthold;M. Listgarten
从成人和青少年牙周炎的人类病变中分离出9株能动的、厌氧的、非芽孢形成的革兰氏阴性细菌，并与模式菌株Fusobacterium planti ATCC 29863、生痰月单胞菌ATCC 33150、反刍月单胞菌ATCC 12561和Pectinatus galliphilus ATCC 29359进行比较。我们的分离株的细胞是长的和蛇形的，似乎是双边鞭毛时，他们通过暗视野显微镜检查，给细胞蜈蚣样的外观。电子显微镜研究表明，存在一个线性区的鞭毛插入螺旋周围的细胞体。培养物是发酵的，并产生丙酸、乙酸、乳酸和琥珀酸。鸟嘌呤加胞嘧啶含量的脱氧核糖核酸的三个分离物的范围从52至54摩尔%，由热变性法测定。所有菌株之间的脱氧核糖核酸同源性为58%至100%，但与先前描述的三个物种的脱氧核糖核酸的同源性仅为14%或更低。我们建议将其命名为牙周鹅不食草属，sp. nov.;菌株LL 2383（= ATCC 35019）是模式菌株。
Nine strains of motile, anaerobic, nonsporeforming, gram-negative bacteria were isolated from human lesions of adult and juvenile periodontitis and compared with type strains Fusobacterium plauti ATCC 29863, Selenomonas sputigena ATCC 33150, Selenomonas ruminantium ATCC 12561, and Pectinatus cerevisiiphilus ATCC 29359. The cells of our isolates were long and serpentine and appeared to be bilaterally flagellated when they were examined by dark-field microscopy, giving the cells a centipede-like appearance. Electron microscopic studies showed the presence of a linear zone of flagellar insertions which spiraled around the cell body. Cultures were fermentative and produced propionic, acetic, lactic, and succinic acids. The guanine-plus-cytosine contents of the deoxyribonucleic acids of three isolates ranged from 52 to 54 mol%, as determined by the thermal denaturation method. All strains had 58 to 100% deoxyribonucleic acid homology with one another, but only 14% or less homology with the deoxyribonucleic acids of three previously described species. We propose the name Centipeda periodontii gen. nov., sp. nov. for these organisms; strain LL2383 (= ATCC 35019) is the type strain.