Diurnal dynamic behavior of microglia in response to infected bacteria through the UDP-P2Y6 receptor system.
Diurnal dynamic behavior of microglia in response to infected bacteria through the UDP-P2Y6 receptor system.
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DOI：
10.1038/srep30006
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发表时间：
2016-07-21
影响因子：
4.6
通讯作者：
Nakanishi H
中科院分区：
文献类型：
--
作者：
Takayama F;Hayashi Y;Wu Z;Liu Y;Nakanishi H
长期以来，人们一直认为，当细菌感染到脑实质时，小胶质细胞通过缩回它们的长突起和消耗病原体而在形态上转变为活化状态。然而，在本研究中，我们首次表明，小鼠皮质小胶质细胞延长其进程对局灶性注射牙龈卟啉单胞菌。这种牙龈卟啉单胞菌诱导的小胶质细胞突起延伸在光（睡眠）阶段比黑暗（清醒）阶段显著增加。与此相反，局灶性注射ATP诱导的小胶质细胞突起延伸显着增加，在黑暗阶段比光相。此外，与ATP诱导的小胶质细胞突起延伸的P2Y12受体介导的机制相反，牙龈卟啉单胞菌介导的小胶质细胞突起延伸是由P2Y6受体介导的。细菌如牙龈卟啉单胞菌感染脑实质可诱导感染部位的小胶质细胞分泌UDP，这反过来又诱导邻近小胶质细胞的突起延伸。
It has long been believed that microglia morphologically transform into the activated state by retracting their long processes and consuming pathogens when bacteria infect into the brain parenchyma. In the present study, however, we showed for the first time that murine cortical microglia extend their processes towards focally injected Porphyromonas gingivalis. This P. gingivalis-induced microglial process extension was significantly increased during the light (sleeping) phase than the dark (waking) phase. In contrast, focally injected ATP-induced microglial process extension was significantly increased during the dark phase than the light phase. Furthermore, in contrast to the P2Y12 receptor-mediated mechanism of ATP-induced microglial process extension, the P. gingivalis-mediated microglial process extension was mediated by P2Y6 receptors. The infection of bacteria such as P. gingivalis to the brain parenchyma may induce the secretion of UDP from microglia at the site of infection, which in turn induces the process extension of the neighboring microglia.