Reproductive Toxicity and Teratogenicity of Fluorene-9-bisphenol on Chinese Medaka (Oryzias sinensis): A Study from Laboratory to Field
Reproductive Toxicity and Teratogenicity of Fluorene-9-bisphenol on Chinese Medaka (Oryzias sinensis): A Study from Laboratory to Field
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DOI：
10.1021/acs.est.2c05753
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发表时间：
2022-11-28
影响因子：
11.4
通讯作者：
Zhang, Zhaobin
中科院分区：
文献类型：
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作者：
Fan, Xiaolin;Guo, Jilong;Zhang, Zhaobin
作为双酚a （BPA）的替代品，芴-9-双酚（BHPF）越来越多地被用作合成聚合物的材料，广泛应用于道路标线、人造轨道、地板涂料、建筑涂料等领域，由于产品释放出BHPF，导致水生环境污染的可能性增加。然而，到目前为止，尚不清楚它是否会对真实环境中的鱼类产生实际影响。本研究在实验室条件下对中华米鳉鱼（Oryzias sinensis）进行了为期105天的不同浓度（0.01、0.1、1和10 μ g/ L） BHPF暴露实验，发现其繁殖能力下降，表现为卵子质量和数量下降，卵发生迟缓，卵泡闭锁，F1代鱼眼和体变形。毒性转录组分析显示，BHPF显著抑制了雌激素应答基因，表明BHPF对雌激素受体的拮抗作用可能是导致繁殖力下降的原因。实地调查（北京）表明，60%的地表水检测到BHPF，气相色谱-质谱法检测到BHPF的平均浓度为10.49 +/- 6.33 ng/L，在野生中华medaka中也观察到类似的影响，其中一些参数与相应水体BHPF水平有明显的相关性。
Fluorene-9-bisphenol (BHPF), a bisphenol A (BPA) substitute, has been increasingly used as a material in syntheses of polymers that are widely used in road markings, artificial tracks, coating floors, building paints, etc., increasing the likelihood of BHPF contamination in the aquatic environment due to its release from the products. However, to date, it is unknown whether it may have actual impacts on fish in real environments. In this study, a 105-day exposure experiment of BHPF at various concentrations (0.01, 0.1, 1, and 10 mu g/ L) on Chinese medaka (Oryzias sinensis) was performed under laboratory conditions and found decreased fecundity, such as lower egg qualities and quantities, retarded oogenesis, and atretic follicles in the fish and deformed eyes and bodies in its F1 generation. Toxicotranscriptome analyses showed that estrogen-responsive genes were significantly suppressed by BHPF, indicating that antagonist properties of BHPF on estrogen receptors might be causes for the decreased fecundity. Field investigations (Beijing) demonstrated that BHPF was detectable in 60% surface waters, with a mean concentration of 10.49 +/- 6.33 ng/L, by gas chromatography-mass spectrometry, and similar effects in wild Chinese medaka were also observed, some of which the parameters were found to be obviously correlated with the BHPF levels in corresponding waters.