CALHM1/CALHM3 channel is intrinsically sorted to the basolateral membrane of epithelial cells including taste cells
CALHM1/CALHM3 channel is intrinsically sorted to the basolateral membrane of epithelial cells including taste cells
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DOI：
10.1038/s41598-019-39593-5
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发表时间：
2019-02-25
影响因子：
4.6
通讯作者：
Taruno, Akiyuki
中科院分区：
文献类型：
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作者：
Kashio, Makiko;Gao Wei-qi;Taruno, Akiyuki
极化味觉细胞基底外侧膜中的 CALHM1/CALHM3 通道介导神经递质释放。然而，调节其定位的机制仍有待探索。在这里，我们在靠近传入神经纤维的离散点中的 II 型味觉细胞的基底外侧膜中发现了 CALHM1/CALHM3。与味觉细胞一样，CALHM1/CALHM3 存在于模型上皮细胞的基底外侧膜中，尽管它分布在整个膜上并且没有显示出在斑点中的积累。我们在 CALHM1 和 CALHM3 中鉴定了典型的基底外侧分选信号：基于酪氨酸和双亮氨酸的基序。然而，在缺乏这些信号的突变通道中，基底外侧排序保持完整，这表明非规范信号存在于其他地方。我们的研究证明了极化细胞中 CALHM 通道的内在基底外侧排序，并提供了机制见解。
The CALHM1/CALHM3 channel in the basolateral membrane of polarized taste cells mediates neurotransmitter release. However, mechanisms regulating its localization remain unexplored. Here, we identified CALHM1/CALHM3 in the basolateral membrane of type II taste cells in discrete puncta localized close to afferent nerve fibers. As in taste cells, CALHM1/CALHM3 was present in the basolateral membrane of model epithelia, although it was distributed throughout the membrane and did not show accumulation in puncta. We identified canonical basolateral sorting signals in CALHM1 and CALHM3: tyrosine-based and dileucine motifs. However, basolateral sorting remained intact in mutated channels lacking those signals, suggesting that non-canonical signals reside elsewhere. Our study demonstrates intrinsic basolateral sorting of CALHM channels in polarized cells, and provides mechanistic insights.