Chlorophyllase in Piper betle L. has a role in chlorophyll homeostasis and senescence dependent chlorophyll breakdown
Chlorophyllase in Piper betle L. has a role in chlorophyll homeostasis and senescence dependent chlorophyll breakdown
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DOI：
10.1007/s11033-012-1545-8
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发表时间：
2012-06-01
影响因子：
2.8
通讯作者：
Kumar, Nikhil
中科院分区：
文献类型：
--
作者：
Gupta, Supriya;Gupta, Sanjay Mohan;Kumar, Nikhil
胡椒鲜叶叶绿素含量和叶绿素酶活性的3.1.1.14长白猪KS分别比KV高2倍和低8倍，叶绿素与叶绿素酶活性呈负相关。比叶绿素酶活性是近8倍以上，在KV比KS。在KV和KS的叶绿素酶cDNA中发现了918 nt的开放阅读框。该基因在两个地方品种中都以单拷贝存在。编码的306个氨基酸的多肽在KV和KS之间仅在两个位置不同; 203（半胱氨酸到酪氨酸）和301（谷氨酰胺到甘氨酸）。在鲜叶和离体叶片中，KV和KS的叶绿素酶基因表达存在明显差异。叶绿素酶基因在两个地方品种中都被阿坝上调，而被BAP下调，但在KV和KS之间存在数量上的差异。结果表明，叶绿素酶参与了甜菜采后衰老过程中叶绿素的动态平衡和叶绿素的损失。
Total chlorophyll content and chlorophyllase (chlorophyll-chlorophyllido hydrolase EC 3.1.1.14) activity in fresh leaves of Piper betle L. landrace KS was, respectively, twofold higher and eight fold lower than KV, showing negative correlation between chlorophyll and chlorophyllase activity. Specific chlorophyllase activity was nearly eightfold more in KV than KS. ORF of 918 nt was found in cloned putative chlorophyllase cDNAs from KV and KS. The gene was present as single copy in both the landraces. The encoded polypeptide of 306 amino acids differed only at two positions between the KV and KS; 203 (cysteine to tyrosine) and 301 (glutamine to glycine). Difference in chlorophyllase gene expression between KV and KS was evident in fresh and excised leaves. Up regulation of chlorophyllase gene by ABA and down regulation by BAP was observed in both the landraces; however, there was quantitative difference between KV and KS. Data suggests that chlorophyllase in P. betle is involved in chlorophyll homeostasis and chlorophyll loss during post harvest senescence.