On the use of trace levels of [1-14 C] galactose to estimate cycling between fructose 6-phosphate and fructose diphosphate.
On the use of trace levels of [1-14 C] galactose to estimate cycling between fructose 6-phosphate and fructose diphosphate.
复制标题
使用痕量水平的 [1-14 C] 半乳糖来估计果糖 6-磷酸和果糖二磷酸之间的循环。
DOI：
10.1016/0003-9861(82)90186-2
复制
发表时间：
1982
影响因子：
3.9
通讯作者：
Blum,JJ
中科院分区：
文献类型：
--
作者：
Crawford,JM;Blum,JJ
[1-14C]半乳糖法测定肝制剂中磷酸果糖激酶通量的研究[j]。物化学。生物物理学。177,337-345)已经用计算机程序进行了评估，该程序计算了代谢系统中所有中间体的所有碳原子的14c标签流量。我们的计算表明，如果醛缩酶的双向通量至少是糖异生速率的两倍，那么在没有糖原分解或外源葡萄糖磷酸化的情况下，由二羟丙酮进行糖异生时，该方法的误差相对较小。如果实验系统偏离这些条件，可能会引入磷酸果糖激酶通量的估计。
The [1-14C]galactose method for estimating phosphofructokinase flux in liver preparations (R. Rognstad and J. Katz, 1976,Arch. Biochem. Biophys.177, 337–345) has been evaluated using a computer program that calculates the flow of14C label through all the carbon atoms of all the intermediates in a metabolic system. Our computations show that this method is subject to relatively small errors when gluconeogenesis from dihydroxyacetone proceeds in the absence of glycogenolysis or of phosphorylation of exogenous glucose, provided that bidirectional flux through aldolase is at least twice the rate of gluconeogenesis. Significant error may be introduced into estimates of phosphofructokinase flux if the experimental system deviates from these conditions.