Comparative transcriptome analysis reveals the function of SlPRE2 in multiple phytohormones biosynthesis, signal transduction and stomatal development in tomato
Comparative transcriptome analysis reveals the function of SlPRE2 in multiple phytohormones biosynthesis, signal transduction and stomatal development in tomato
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DOI：
10.1007/s00299-023-03001-0
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发表时间：
2023-04
影响因子：
6.2
通讯作者：
Zhiguo Zhu;Menglin Luo;Jialing Li;Baolu Cui;Zixin Liu;Dapeng Fu;Huiwen Zhou;Anpei Zhou
中科院分区：
文献类型：
--
作者：
Zhiguo Zhu;Menglin Luo;Jialing Li;Baolu Cui;Zixin Liu;Dapeng Fu;Huiwen Zhou;Anpei Zhou
多效唑抗性蛋白（Pac lobutrazol resistance proteins，PREs）是一类碱性/螺旋-环-螺旋（basic/helix-loop-helix，bHLH）转录因子家族的非典型成员，在植物生长发育过程中，通过不同的植物激素调节植物的形态、细胞大小、色素代谢和非生物胁迫。然而，对PREs在番茄生长发育中的网络调控机制知之甚少。本研究探讨了SlPRE 2在番茄生长发育中的作用及其机制。定量RT-PCR结果表明，SlPRE 2的表达受多种激素和非生物胁迫的调控。它在光周期中表现为光抑制表达。RNA-seq结果显示，SlPRE 2调控的基因涉及光合作用、叶绿素代谢、激素代谢和信号传导、碳水化合物代谢等多个方面，表明SlPRE 2在赤霉素、油菜素类固醇、生长素、细胞分裂素、脱落酸和水杨酸等激素调节植物发育过程中发挥作用。此外，SlPRE 2过表达植株在幼叶中表现出广泛开放的气孔，并且参与气孔发育的四个基因表现出表达改变。这些结果揭示了SlPRE 2调控植物激素和胁迫反应的机制，揭示了SlPRE 2在番茄气孔发育中的作用。这些发现为进一步了解SlPRE 2调控番茄生长发育的分子机制提供了有用的线索。
Key messageTranscriptomic, physiological, and qRT-PCR analysis revealed the potential mechanism by which SlPRE2 regulates plant growth and stomatal size via multiple phytohormone pathways in tomato.AbstractPaclobutrazol resistance proteins (PREs) are atypical members of the basic/helix-loop-helix (bHLH) transcription factor family that regulate plant morphology, cell size, pigment metabolism and abiotic stress in response to different phytohormones. However, little is known about the network regulatory mechanisms of PREs in plant growth and development in tomato. In this study, the function and mechanism ofSlPRE2in tomato plant growth and development were investigated. The quantitative RT-PCR results showed that the expression ofSlPRE2was regulated by multiple phytohormones and abiotic stresses. It showed light-repressed expression during the photoperiod. The RNA-seq results revealed thatSlPRE2regulated many genes involved in photosynthesis, chlorophyll metabolism, phytohormone metabolism and signaling, and carbohydrate metabolism, suggesting the role ofSlPRE2in gibberellin, brassinosteroid, auxin, cytokinin, abscisic acid and salicylic acid regulated plant development processes. Moreover,SlPRE2overexpression plants showed widely opened stomata in young leaves, and four genes involved in stomatal development showed altered expression. Overall, the results demonstrated the mechanism by whichSlPRE2regulates phytohormone and stress responses and revealed the function ofSlPRE2in stomatal development in tomato. These findings provide useful clues for understanding the molecular mechanisms ofSlPRE2-regulated plant growth and development in tomato.