Transretinal electrical stimulation with a suprachoroidal multichannel electrode in rabbit eyes
Transretinal electrical stimulation with a suprachoroidal multichannel electrode in rabbit eyes
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DOI：
10.1007/s10384-004-0055-1
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发表时间：
2004-05-01
影响因子：
2.4
通讯作者：
Tano, Y
中科院分区：
文献类型：
--
作者：
Sakaguchi, H;Fujikado, T;Tano, Y
目的：之前已经提出了几种用于放置视觉假体的电极装置的方法。在这项研究中，我们调查，如果从脉络膜上腔经视网膜刺激可以引起一个电诱发电位（EEP）在白化病兔。方法：平面电极阵列（聚酰亚胺板，铂电极）的发展和用于这项研究。在距利姆布斯2-2.5 mm处进行巩膜切口并放置锚定缝合线后，通过在显微镜下直接观察将阵列插入眼睛后部的脉络膜上腔中。将铂丝植入玻璃体腔作为参比电极。对于电刺激，使用双相脉冲。结果：从脉络膜上腔电刺激时，硬膜外电极可记录到EEP，其阈值为66.0 ± 32.1 μ A（42.0 μ C/cm ~ 2）。组织学检查表明，没有重大损害的视网膜和脉络膜的插入和放置的阵列和电stimulation.Conclusions：从脉络膜上腔的经视网膜电刺激可以引起EEP，这表明这种方法可能是有用的视网膜假体系统。(C)日本眼科学会2004年。
Purpose: Several approaches for placing an electrode device for visual prosthesis have been previously proposed. In this study, we investigated if transretinal stimulation from the suprachoroidal space can elicit an electrical evoked potential (EEP) in albino rabbits.Methods: A flat electrode array (polyimide plate, platinum electrode) was developed and used for this study. After performing a scleral incision at 2-2.5 mm from the limbus and placing an anchoring suture, the array was inserted into the suprachoroidal space in the posterior portion of the eye by direct observation under a microscope. A platinum wire was implanted into the vitreous space as a reference electrode. For electrical stimulation, a biphasic pulse was used. When the electrode was stimulated, the EEP was recorded.Results: When the electrical stimulation from the suprachoroidal space was applied, the EEP could be recorded with an epidural electrode, and the threshold was 66.0 +/- 32.1 muA (42.0 muC/cm(2)). Histological examination indicated the absence of major damage to the retina and choroid from the insertion and placement of the array and the electrical stimulation.Conclusions: Transretinal electrical stimulation from the suprachoroidal space could elicit EEP, suggesting that this approach may be useful for a retinal prosthesis system. (C) Japanese Ophthalmological Society 2004.