SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box
SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box
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DOI：
10.1016/s0092-8674(00)80098-7
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发表时间：
1996-07-26
期刊：
影响因子：
64.5
通讯作者：
Elledge, SJ
中科院分区：
文献类型：
--
作者：
Bai, C;Sen, P;Elledge, SJ
我们已经确定了酵母和人类同源的SKP 1基因作为一个抑制cdc 4突变体和细胞周期蛋白F结合蛋白。Skp 1 p通过Skp 2 p间接结合细胞周期蛋白A/Cdk 2，并通过称为F-box的新型结构基序直接结合Skp 2 p、细胞周期蛋白F和Cdc 4p。SKP 1是泛素介导的Cln 2 p、Clb 5 p和Cdk抑制剂Sic 1 p的蛋白水解所必需的，并提供了这些分子与蛋白水解机制之间的联系。大量的蛋白质含有F-box基序，从而参与泛素途径。不同的skp 1突变体逮捕细胞在G1或G2，这表明调节蛋白质水解在不同阶段的周期之间的连接。
We have identified the yeast and human homologs of the SKP1 gene as a suppressor of cdc4 mutants and as a cyclin F-binding protein. Skp1p indirectly binds cyclin A/Cdk2 through Skp2p, and directly binds Skp2p, cyclin F, and Cdc4p through a novel structural motif called the F-box. SKP1 is required for ubiquitin-mediated proteolysis of Cln2p, Clb5p, and the Cdk inhibitor Sic1p, and provides a link between these molecules and the proteolysis machinery. A large number of proteins contain the F-box motif and are thereby implicated in the ubiquitin pathway. Different skp1 mutants arrest cells in either G1 or G2, suggesting a connection between regulation of proteolysis in different stages of the cycle.