Disposable lateral flow-through strip for smartphone-camera to quantitatively detect alkaline phosphatase activity in milk
Disposable lateral flow-through strip for smartphone-camera to quantitatively detect alkaline phosphatase activity in milk
复制标题
用于智能手机相机的一次性横向流通条，用于定量检测牛奶中的碱性磷酸酶活性
DOI：
10.1016/j.bios.2015.02.035
复制
发表时间：
2015-07-15
影响因子：
12.6
通讯作者：
Li, ChangMing
中科院分区：
文献类型：
--
作者：
Yu, Ling;Shi, ZhuanZhuan;Li, ChangMing
A disposable lateral flow-through strip was developed for smartphone to fast one-step quantitatively detect alkaline phosphatase (ALP) activity in raw milk. The strip comprises two functional components, a conjugation pad loaded with phosphotyrosine-coated gold nanoparticles (AuNPs@Cys-Try-p) and a testing line coated with anti-phosphotryosine antibody (anti-Tyr-p mAb). The dephosphorylation activity of ALP at the testing zone can be quantitatively assayed by monitoring the accumulated AuNPs-induced color changes by smartphone camera, thus providing a highly convenient portable detection method. A trace amount of ALP as low as 0.1 U L-1 with a linear dynamic range of 0.1-150 U L-1 (R-2=0.999) in pasteurized milk and raw milk can be one-step detected by the developed flow-through strip within 10 min, demonstrating the potential of smartphone-based portable sensing device for pathogen detection. This bio-hazards free lateral flow-through testing strip can be also used to fabricate rapid, sensitive and inexpensive enzyme or immunosensors for broad portable clinic diagnosis and food contamination analysis, particularly in point-of-care and daily food quality inspection. (C) 2015 Elsevier B.V. All rights reserved.
