Role of specific lipids and annexins in calcium-dependent membrane fusion.
Role of specific lipids and annexins in calcium-dependent membrane fusion.
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特定脂质和膜联蛋白在钙依赖性膜融合中的作用。
DOI：
10.1111/j.1749-6632.1991.tb36497.x
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发表时间：
1991
影响因子：
5.2
通讯作者：
Papaphadjopoulos,D
中科院分区：
文献类型：
--
作者：
Meers,P;Hong,KL;Papaphadjopoulos,D
细胞内Ca2+的浓度在细胞分泌和其他膜融合事件中受到高度调节。因此，Ca2+可能直接或间接地在这些过程中起一定作用。Ca2+在膜融合中的直接作用的一个早期迹象是观察到Ca2+可以诱导由最丰富的天然酸性磷脂磷脂酰丝氨酸（PS）组成的模型膜囊的聚集和内容物泄漏。“后来这些现象被证明与囊泡融合有关。随后，产生大单层囊泡（LUV） b> '的能力和荧光融合试验的发展，使得对介导Ca2+依赖性膜融合的因素，特别是PS囊泡的融合进行了详细的研究。^膜融合动力学的简单质量作用模型，可以描述或预测由此类研究产生的某些条件下融合试验的结果。该模型的一个突出特点是，整个融合过程被视为一个两步反应，首先是囊泡聚集，然后是实际融合。使用这个合理的框架，每个步骤中明确涉及的因素可以单独讨论。
The concentration of intracellular Ca2+ is highly regulated during cellular secretion and other membrane fusion events. Therefore, it is thought that Ca2+ may play some role, direct or indirect, in these processes. One early indication of a direct role in membrane fusion was the observation that Ca2+ could induce aggregation and leakage of contents from model membrane vesicles composed of the most abundant naturally occurring acidic phospholipid, phosphatidylserine (PS).'Later these phenomena were shown to be associated with fusion of the vesicles. 2 Subsequently, the ability to produce large unilamellar vesicles (LUV)>'and the development of fluorescent fusion assays576 has allowed detailed studies of the factors that mediate Ca2+-dependent membrane fusion, particularly fusion of PS vesicle^.^ A simple mass action model for the kinetics of membrane fusion that could describe or predict the results of fusion assays under certain conditions resulted from such studies.','A prominent feature of this model was that the overall fusion process was treated as a two-step reaction involving first aggregation of vesicles followed by actual fusion. Using this rational framework, the factors explicitly involved at each step can be discussed separately.