An ultrastructural study of the surface and attachment structures of Paradiplozoon homoion (Bychowsky & Nagibina, 1959) (Monogenea: Diplozoidae).
An ultrastructural study of the surface and attachment structures of Paradiplozoon homoion (Bychowsky & Nagibina, 1959) (Monogenea: Diplozoidae).
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DOI：
10.1186/s13071-017-2203-8
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发表时间：
2017-05-25
影响因子：
3.2
通讯作者：
Gelnar M
中科院分区：
文献类型：
--
作者：
Konstanzová V;Koubková B;Kašný M;Ilgová J;Dzika E;Gelnar M
Palombi，1949种（单殖目：复殖虫科）是主要寄生于鲤科鱼类鳃部的吸血性体外寄生虫。虽然这些寄生虫已被许多分类学，系统发育和生态学研究的主题，表面和haptor附着结构的超微结构仍然几乎未知。本研究利用透射电镜观察了双体虫科Paradiplozoonhomoion（Bychowsky & Nagibina，1959）的附着夹和新胚层表面的超微结构，从而拓宽了我们对扁形动物生物学的认识。后体表面有明显的脊状突起，每个脊由几根肌纤维支撑，表面质膜上有鳞片。这种结构以前在双殖虫科的物种中没有记录。对不同身体部位的表面结构进行比较，发现细胞器的厚度和数量略有差异。每个夹子都有一个由骨片组成的扁平碗状结构，骨片是可移动的骨骼状结构，由坚固的、放射状的肌肉束锚定。后中板片基部具腺细胞，腺细胞内有分泌小泡。这项研究为P. homoion的表面和附着钳的超微结构提供了详细的数据，并为Diplozoidae的超微结构提供了新的见解。在二倍体动物中，首次观察到了负责片晶发育的附着夹基部的腺细胞。我们的研究结果支持的假设，特别是新胚层隔室的结构是相似的扁形动物。另一方面，二倍体腺体系统和骨片发育的机制显然值得进一步关注。
Species of Diplozoon Palombi, 1949 (Monogenea: Diplozoidae) are blood-feeding ectoparasites mainly parasitising the gills of cyprinid fishes. Although these parasites have been the subject of numerous taxonomic, phylogenetic and ecological studies, the ultrastructure of the surface and haptor attachment structures remains almost unknown. In this study, we used transmission electron microscopy to examine the ultrastructure of attachment clamps and neodermal surface of Paradiplozoon homoion (Bychowsky & Nagibina, 1959), family Diplozoidae Palombi, 1949, thereby broadening our knowledge of platyhelminth biology. The hindbody surface of P. homoion is distinctly ridged, each ridge being supported by several muscle fibers and equipped with scales on the surface plasma membrane. Such structures have not been recorded previously in species of the family Diplozoidae. Comparisons of the surface structure of different body parts revealed slight differences in the thickness and number of organelles. Each of the clamps has a flattened bowl-like structure composed of sclerites, movable skeletal-like structures that are anchored by robust, radially oriented muscle bundles. The base of the posterior median plate sclerites is equipped with glandular cells possessing secretory vesicles. This study brings detailed ultrastructural data for the surface and haptoral attachment clamps of P. homoion and provides new insights into the ultrastructure of Diplozoidae. Glandular cells at the base of the attachment clamps responsible for sclerite development in diplozoid species were observed for the first time. Our findings support the hypothesis that the structure of particular neodermal compartments is similar within the Platyhelminthes. On the other hand, the diplozoid glandular system and the mechanism of sclerite development clearly merits further attention.