THE DEGREE OF COUPLING OF NUCLEAR ROTATION IN BINUCLEATE 3T3 CELLS
THE DEGREE OF COUPLING OF NUCLEAR ROTATION IN BINUCLEATE 3T3 CELLS
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DOI：
10.1016/0014-4827(86)90512-4
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发表时间：
1986-09-01
影响因子：
3.7
通讯作者：
ALBRECHTBUEHLER, G
中科院分区：
文献类型：
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作者：
PADDOCK, SW;ALBRECHTBUEHLER, G
为了测试两个相邻核的旋转运动之间是否存在机械耦合，我们制备了双核3T3细胞，并通过近红外显微镜观察其核运动，并用延时视频技术记录它们。我们发现 110 个选定的双核细胞中有 49 个 (44%) 表达核旋转。旋转可能仅发生在其中一个核中，而第二个核保持静止 (31/110) 或同时发生在两个核中 (18/110)。在几乎所有两个原子核同时旋转（15/110）的情况下，它们以不同的速度和相反的方向旋转。仅在三个示例中观察到原子核沿相同方向旋转。结果与旋转核与其邻近核之间的弱机械相互作用一致。与我们之前在单核细胞中的观察一致，我们没有在具有旋转核的双核细胞中发现中心球的特征位置或微管或中间丝的特殊分布。在旋转过程中，即使在具有一个旋转核和一个静止核的细胞中旋转核下方的局部区域中，也不存在长的、形状良好的微丝束。也与单核细胞的观察一致，用秋水酰胺处理抑制核旋转，尽管当用正常培养基替换含秋水酰胺的培养基时细胞核旋转的能力最终恢复。
In order to test the existence of mechanical coupling between the rotational movements of two adjacent nuclei, we prepared binucleate 3T3 cells and observed their nuclear movements by near infrared microscopy and recorded them with time-lapse video techniques. We found that 49 out of 110 (44%) of the selected binucleate cells expressed nuclear rotation. Rotation could occur in just one of the nuclei while the second nucleus remained stationary (31/110) or in both nuclei stimultaneously (18/110). In almost all cases where both nuclei rotated simultaneously (15/110) they did so at different speeds and in opposite directions. The nuclei were observed to rotate in the same direction in only three of the examples. The results are consistent with a weak mechanical interaction between a rotating nucleus and its neighbor. Consistent with our previous observations in mononucleate cells, we did not find a characteristic position of the centrosphere or a special distribution of the microtubles or the intermediate filaments in binucleate cells with rotating nuclei. There was an absence of long, well-formed microfilament bundles beneath the nuclei during rotation, even in the local region beneath the rotating nucleus in those cells with one rotating and one stationary nucleus. Also consistent with observations of mononucleate cells, nuclear rotation was inhibited by treatment with colcemid, although the ability of the nuclei to rotate was eventually restored when the colcemid-containing medium was replaced with normal medium.