Qscore: An algorithm for evaluating SEQUEST database search results
Qscore: An algorithm for evaluating SEQUEST database search results
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DOI：
10.1016/s1044-0305(02)00352-5
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发表时间：
2002-04-01
影响因子：
3.2
通讯作者：
Lee, TD
中科院分区：
文献类型：
--
作者：
Moore, RE;Young, MK;Lee, TD
描述了一种评分程序，用于测量使用Sequest数据库搜索程序从MS/MS数据的光谱匹配获得的蛋白质鉴定结果的质量。评分系统基本上是概率性的，并且通过估计蛋白质鉴定偶然发生的概率来操作。该概率基于从蛋白质中鉴定的肽的数量、鉴定的肽的总数以及来自数据库的存在于鉴定的蛋白质中的不同胰蛋白酶肽的分数。该分数不是严格意义上的概率，因为它还包含了有关单个肽匹配质量的信息。在大的测试数据集上使用Qscore的结果与使用验证单个光谱匹配的方法的acl-开发的结果相似，在所识别的蛋白质和假阳性匹配之间的分数仅具有窄的重叠。与已发表的评估Sequest结果的方法直接比较，Qscore能够识别相同数量的蛋白质，而没有任何可识别的假阳性分配。Qscore大大减少了必须手动验证的Sequest蛋白质鉴定的数量。(C)2002年美国质谱学会。
A scoring procedure is described for measuring the quality of the results for protein identifications obtained from spectral matching of MS/MS data using the Sequest database search program. The scoring system is essentially probabilistic and operates by estimating the probability that a protein identification has come about by chance. The probability is based on the number of identified peptides from the protein, the total number of identified peptides, and the fraction of distinct tryptic peptides from the database that are present in, the identified protein. The score is not strictly a probability, as it also incorporates information about the quality of the individual peptide matches. The result of using Qscore on a large test set of data was similar to that acl-deved using approaches that validate individual spectral matches, with only a narrow overlap in scores between identified proteins and false positive matches. In direct comparison with a published method of evaluating Sequest results, Qscore was able to identify an equivalent number of proteins without any identifiable false positive assignments. Qscore greatly reduces the number of Sequest protein identifications that have to be validated manually. (C) 2002 American Society for Mass Spectrometry.