POLYNUCLEOTIDE CROSS-LINKING BY ALUMINUM
POLYNUCLEOTIDE CROSS-LINKING BY ALUMINUM
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DOI：
10.1016/0162-0134(89)85001-9
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发表时间：
1989-12-01
影响因子：
3.9
通讯作者：
EICHHORN, GL
中科院分区：
文献类型：
--
作者：
KARLIK, SJ;EICHHORN, GL
阿尔采默氏病、关岛-帕金森病和肌萎缩性侧索硬化症患者的脑中铝浓度增加，并且铝在染色质中有明显的定位，这一观察结果导致了对铝（III）与DNA相互作用的研究。我们以前已经表明，铝交联小牛胸腺DNA在低pH值（S。J. Karlik，G. L. Eichhorm，P. N.刘易斯和D. R.糟糕Biochemistry 19，5991 [1980]）。扩展的研究表明，交联发生在所有碱基比例的DNA中，包括polyAdT和polyGdC。由于Al交联阻止polydAdT中的复性，因此在Al存在下复性的聚合物的量的减少成为交联程度的定量评价。交联的饱和发生在0.4比的铝核苷酸磷酸，表明潜在的80%的碱基对是铝结合。交联在升高的pH下和通过EDTA被破坏。
The observations that there was an increased concentration of Al in the brains of Alzheimer''s, Guam-Parkinson, and amyotrophic lateral sclerosis disease patients and that there was an apparent localization of the Al in chromatin led to a study of the interaction of Al(III) with DNA. We have previously shown that Al cross-links calf thymus DNA at low pH (S. J. Karlik, G. L. Eichhorm, P. N. Lewis, and D. R. Crapper. Biochemistry 19, 5991 [1980]). Extended studies indicate that cross-linking occurs in DNAs of all base ratios, including polyAdT and polyGdC. Since Al cross-links prevent renaturation in polydAdT, the decrease in the amount of polymer renatured in the presence of Al becomes a quantitative appraisal of the extent of cross-linking. Saturation of cross-linking occurs at a 0.4 ratio of Al to nucleotide phosphate, indicating that potentially 80% of the base pairs are Al bound. Cross-links are broken at elevated pH and by EDTA.