Modulatory effects of norepinephrine, acting on alpha1 receptors in the central nucleus of the amygdala, on behavioral and neuroendocrine responses to acute immobilization stress
Modulatory effects of norepinephrine, acting on alpha1 receptors in the central nucleus of the amygdala, on behavioral and neuroendocrine responses to acute immobilization stress
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DOI：
10.1016/s0028-3908(02)00292-7
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发表时间：
2002-12-01
影响因子：
4.7
通讯作者：
Morilak, DA
中科院分区：
文献类型：
--
作者：
Cecchi, M;Khoshbouei, H;Morilak, DA
杏仁核中央核 (CeA) 是边缘恐惧-焦虑回路的组成部分，也与下丘脑-垂体-肾上腺 (HPA) 应激轴的调节有关。 CeA 接受密集的去甲肾上腺素能神经支配，并且富含 α(1)-肾上腺素能受体的表达。我们假设去甲肾上腺素 (NE) 作用于 CeA 中的 α(1) 受体，可能调节压力引起的焦虑样行为反应和 HPA 激活。为了研究 CeA 中 α(1) 肾上腺素能受体对应激诱导行为反应的作用，将 α(1) 拮抗剂 Benoxathian 显微注射到雄性 Sprague-Dawley 大鼠的两侧 CeA 中，并在社交互动 (SI) 测试和高架十字迷宫 (EPMZ) 上测量对急性固定应激的焦虑样行为反应。 Benoxathian 剂量依赖性地阻断由固定应激引起的 SI 时间的缩短，而 β 受体拮抗剂则没有效果，这与 CeA 中不存在 β 受体一致。相比之下，在单独的实验中，benoxathian 对应激引起的 EPMZ 开臂探索行为的减少没有影响，对应激引起的血浆 ACTH 分泌也没有影响。这些结果证实，SI 测试和 EPMZ 测量可以独立调节的行为应激反应性的不同方面，同样，行为应激反应性的去甲肾上腺素能调节可以独立于 HPA 轴的调节而发生。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
The central nucleus of the amygdala (CeA) is a component of the limbic fear-anxiety circuit, and has also been implicated in regulation of the hypothalamic-pituitary-adrenal (HPA) stress axis. The CeA receives dense noradrenergic innervation, and is rich in expression of alpha(1)-adrenergic receptors. We hypothesized that norepinephrine (NE), acting on alpha(1) receptors in CeA, may modulate stress-induced anxiety-like behavioral responses and HPA activation. To investigate the role of alpha(1) adrenergic receptors in CeA on stress-induced behavioral reactivity, the alpha(1) antagonist benoxathian was microinjected bilaterally into CeA of male Sprague-Dawley rats, and anxiety-like behavioral responses to acute immobilization stress were measured on the Social Interaction (SI) test and on the Elevated Plus-maze (EPMZ). Benoxathian dose dependently blocked the reduction in SI time induced by immobilization stress, whereas beta-receptor antagonists had no effect, consistent with an absence of beta-receptors in CeA. By contrast, in separate experiments, benoxathian had no effect on stress-induced reduction in open-arm exploratory behavior on the EPMZ, nor on stress-induced plasma ACTH secretion. These results confirm that the SI test and EPMZ measure different aspects of behavioral stress reactivity that can be modulated independently, and likewise, that noradrenergic modulation of behavioral stress reactivity can occur independently of modulation of the HPA axis. (C) 2002 Elsevier Science Ltd. All rights reserved.