ZINC-CONTAINING 7S-NGF COMPLEX - EVIDENCE FROM ZINC HISTOCHEMISTRY FOR LOCALIZATION IN SALIVARY SECRETORY GRANULES
ZINC-CONTAINING 7S-NGF COMPLEX - EVIDENCE FROM ZINC HISTOCHEMISTRY FOR LOCALIZATION IN SALIVARY SECRETORY GRANULES
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DOI：
10.1177/35.5.2435783
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发表时间：
1987-05-01
影响因子：
3.2
通讯作者：
DANSCHER, G
中科院分区：
文献类型：
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作者：
FREDERICKSON, CJ;PEREZCLAUSELL, J;DANSCHER, G
7S-NGF是一种含有神经营养亚基β-NGF的前体蛋白，通过免疫细胞化学定位于某些小鼠唾液腺颗粒曲管(GCT)细胞颗粒[Watson等，Anat Rec(1985)213：365]。7S-NGF前蛋白含有锌，并由锌离子稳定[Pattison和Dunn，生物化学(1976)15：3696]。在本工作中，锌的测定采用了双硫腙、甲苯磺酰胺喹啉和NEO-TIMM‘’S方法，以确定(A)锌组织化学是否可以用来显示与7S-神经生长因子复合体相关的锌，如果可以，(B)锌组织化学是否可以证实7S-神经生长因子复合体在GCT分泌颗粒中的定位。结果表明：(A)锌染色强度随不同唾液腺中NGF水平的不同而不同，以及(B)锌选择性地集中在GCT分泌颗粒中。我们认为锌组织化学可能是锌稳定的7S-NGF蛋白存在的一个有用的标记。
7S-NGF is a pro-protein containing a neurotrophic subunit, beta-NGF, which has been localized by immunocytochemistry to the granules of granular convoluted tubule (GCT) cells in certain murine salivary glands [Watson et al., Anat Rec (1985) 213:365]. The 7S-NGF pro-protein contains zinc and is stabilized by zinc ions [Pattison and Dunn, Biochemistry (1976) 15:3696]. In the present work, dithizone, toluene sulfonamide quinoline (TSQ), and neo-Timm''s methods for zinc were used to determine (a) whether zinc histochemistry could be used to visualize the zinc associated with the 7S-NGF complex and, if so, (b) whether zinc histochemistry might corroborate the reported localization of the 7S-NGF complex in GCT secretory granules. The results indicate (a) that intensity of zinc staining varies with the reported variation in NGF levels in different salivary glands, and (b) that the zinc is selectively concentrated in the GCT secretory granules. We suggest that zinc histochemistry may be a useful marker for the presence of the zinc-stabilized 7S-NGF protein.