Isolation and Identification of Antifungal Compounds from Bacillus subtilis C9 Inhibiting the Growth of Plant Pathogenic Fungi.
Isolation and Identification of Antifungal Compounds from Bacillus subtilis C9 Inhibiting the Growth of Plant Pathogenic Fungi.
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DOI：
10.5941/myco.2012.40.1.059
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发表时间：
2012-03
期刊：
影响因子：
1.9
通讯作者：
Song CH
中科院分区：
文献类型：
--
作者：
Islam MR;Jeong YT;Lee YS;Song CH
分离了拮抗立枯丝核菌的拮抗微生物，并对其抗真菌活性进行了研究。从不同土壤样品中分离到216株细菌，其中19株对所选植物病原真菌有不同程度的拮抗作用。其中，C9菌株被选为一株具有进一步研究潜力的拮抗微生物。用筛选出的C9菌株处理结缕草，显著降低了立枯丝核菌AG2-2(IV)在结缕草上的定殖率，促进了结缕草的生长。经生化、生理和16S rDNA鉴定，该菌株为枯草芽孢杆菌亚种。枯草杆菌。甘露醇(1%)和豆粕(1%)分别是用于抗生素生产的最佳碳源和氮源。从C9菌株发酵液的乙酸乙酯提取物中分离纯化出一种抗菌化合物DG4。根据质子核磁共振谱(~1H核磁共振)、碳核磁共振谱(~(13)C核磁共振谱)和质量分析等波谱数据，确定其化学结构为乙酰丁二醇的立体异构体。C9菌株的乙酸乙酯提取物对几种植物病原菌具有剂量依赖性的抑制作用。用纯化的化合物(乙酰丁二醇的异构体)处理后，受试病原菌的生长受到显著抑制。菌株C9的无细胞培养上清液对几丁质培养基有几丁质酶效应。本研究结果表明，从菌株C9中分离得到的化合物具有作为生防剂和植物生长促进剂的巨大潜力，能够触发植物诱导的系统抗性。
Antagonistic microorganisms against Rhizoctonia solani were isolated and their antifungal activities were investigated. Two hundred sixteen bacterial isolates were isolated from various soil samples and 19 isolates were found to antagonize the selected plant pathogenic fungi with varying degrees. Among them, isolate C9 was selected as an antagonistic microorganism with potential for use in further studies. Treatment with the selected isolate C9 resulted in significantly reduced incidence of stem-segment colonization by R. solani AG2-2(IV) in Zoysia grass and enhanced growth of grass. Through its biochemical, physiological, and 16S rDNA characteristics, the selected bacterium was identified as Bacillus subtilis subsp. subtilis. Mannitol (1%) and soytone (1%) were found to be the best carbon and nitrogen sources, respectively, for use in antibiotic production. An antibiotic compound, designated as DG4, was separated and purified from ethyl acetate extract of the culture broth of isolate C9. On the basis of spectral data, including proton nuclear magneric resonance (1H NMR), carbon nuclear magneric resonance (13C NMR), and mass analyses, its chemical structure was established as a stereoisomer of acetylbutanediol. Application of the ethyl acetate extract of isolate C9 to several plant pathogens resulted in dose-dependent inhibition. Treatment with the purified compound (an isomer of acetylbuanediol) resulted in significantly inhibited growth of tested pathogens. The cell free culture supernatant of isolate C9 showed a chitinase effect on chitin medium. Results from the present study demonstrated the significant potential of the purified compound from isolate C9 for use as a biocontrol agent as well as a plant growth promoter with the ability to trigger induced systemic resistance of plants.