Structural insights into the mechanism of adaptive ribosomal modification by Pseudomonas RimK.
Structural insights into the mechanism of adaptive ribosomal modification by Pseudomonas RimK.
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作者：
细菌拥有多种多样的调节工具，使它们能够迅速适应环境。RimK系统允许假单胞菌通过改变核糖体亚基RpsF的转录后调节来适应。RimK存在于多种细菌中，具有保守的氨基酸序列，然而，其遗传背景和作用是高度多样化的。通过解决和比较两个相关但功能不同的系统的RimK同源物的结构，我们发现了可能导致这些同源物活性水平不同的关键结构差异。此外，我们首次能够清楚地分辨出该蛋白的活性位点，从而分辨出谷氨酸底物的结合。这项工作促进了我们对蛋白质序列和结构的细微差异如何对蛋白质活性产生深远影响的理解，这反过来又会导致广泛的机制变化。
Bacteria are equipped with a diverse set of regulatory tools that allow them to quickly adapt to their environment. The RimK system allows for Pseudomonas spp. to adapt through post‐transcriptional regulation by altering the ribosomal subunit RpsF. RimK is found in a wide range of bacteria with a conserved amino acid sequence, however, the genetic context and the role of this protein is highly diverse. By solving and comparing the structures of RimK homologs from two related but functionally divergent systems, we uncovered key structural differences that likely contribute to the different activity levels of each of these homologs. Moreover, we were able to clearly resolve the active site of this protein for the first time, resolving binding of the glutamate substrate. This work advances our understanding of how subtle differences in protein sequence and structure can have profound effects on protein activity, which can in turn result in widespread mechanistic changes.
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影响因子：
2.1
作者：
Grenga L;Little RH;Malone JG
通讯作者：
Malone JG
DOI：
10.1107/s0907444910045749
发表时间：
2011-04
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Winn MD;Ballard CC;Cowtan KD;Dodson EJ;Emsley P;Evans PR;Keegan RM;Krissinel EB;Leslie AG;McCoy A;McNicholas SJ;Murshudov GN;Pannu NS;Potterton EA;Powell HR;Read RJ;Vagin A;Wilson KS
通讯作者：
Wilson KS
DOI：
10.1107/s2053230x18007689
发表时间：
2018-07-01
影响因子：
0.9
作者：
Arimura, Yasuhiro;Kono, Tomonori;Kurumizaka, Hitoshi
通讯作者：
Kurumizaka, Hitoshi
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
DOI：
10.1007/bf02464894
发表时间：
1989-06-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
KANG, WK;ICHO, T;ISONO, K
通讯作者：
ISONO, K