A copia-like retrotransposon insertion in the upstream region of the SHATTERPROOF1 gene, BnSHP1.A9, is associated with quantitative variation in pod shattering resistance in oilseed rape.
A copia-like retrotransposon insertion in the upstream region of the SHATTERPROOF1 gene, BnSHP1.A9, is associated with quantitative variation in pod shattering resistance in oilseed rape.
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SHATTERPROOF1 基因 BnSHP1.A9 上游区域的复制样逆转录转座子插入与油菜荚果破碎抗性的数量变化相关
DOI：
10.1093/jxb/eraa281
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发表时间：
2020-09-19
影响因子：
6.9
通讯作者：
Hu Q
中科院分区：
文献类型：
--
作者：
Liu J;Zhou R;Wang W;Wang H;Qiu Y;Raman R;Mei D;Raman H;Hu Q
油菜抗裂荚性受SHP1.A9等位基因的影响，该等位基因在启动子区含有copia样反转录转座子插入，其通过DNA甲基化表观遗传地沉默SHP1.A9表达。落荚造成的种子损失是油菜生产中的一个主要制约因素。然而，抗裂荚的分子机制还不清楚。结果表明，数量性状基因座qSRI.A9.1的抗裂性受B基因控制。A9，在由指定为R1和R2的亲本产生的加倍单倍体群体中以及在一组不同的油菜中。双单倍体群体的R1母本亲本系携带qSRI.A9.1处的落粒性等位基因，而R2亲本系携带抗落粒性等位基因。定量RT-PCR结果表明，BnSHP1.A9在R1的花蕾、花和发育中的角果中特异表达，而在R2的任何组织中均不表达。组成型表达来自R1和R2亲本系的BnSHP1.A9等位基因中的任一个的转基因植物表明，这两个等位基因通过促进enb层的木质化的机制负责荚果破碎。这些结果表明，BnSHP1.A9基因本身的等位基因差异不是qSRI.A9.1抗落粒性数量变异的原因。相反，一个高度甲基化copia样长末端重复反转录转座子插入（4803 bp）的启动子区域的R2等位基因的BnSHP1.A9抑制的表达，BnSHP1.A9，从而有助于抗裂荚。最后，我们发现了一个基于copia类反转录转座子的标记BnSHP1.A9R2，可以用于油菜抗裂荚性状的标记辅助育种。
Pod shatter resistance of rapeseed is influenced by a SHP1.A9 allele that contains a copia-like retrotransposon insertion in the promoter region, which silences SHP1.A9 expression epigenetically via DNA methylation. Seed loss resulting from pod shattering is a major constraint in production of oilseed rape (Brassica napus L.). However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here, we show that the pod shatter resistance at quantitative trait locus qSRI.A9.1 is controlled by one of the B. napus SHATTERPROOF1 homologs, BnSHP1.A9, in a doubled haploid population generated from parents designated R1 and R2 as well as in a diverse panel of oilseed rape. The R1 maternal parental line of the doubled haploid population carried the allele for shattering at qSRI.A9.1, while the R2 parental line carried the allele for shattering resistance. Quantitative RT-PCR showed that BnSHP1.A9 was expressed specifically in flower buds, flowers, and developing siliques in R1, while it was not expressed in any tissue of R2. Transgenic plants constitutively expressing either of the BnSHP1.A9 alleles from the R1 and R2 parental lines showed that both alleles are responsible for pod shattering, via a mechanism that promotes lignification of the enb layer. These findings indicated that the allelic differences in the BnSHP1.A9 gene per se are not the causal factor for quantitative variation in shattering resistance at qSRI.A9.1. Instead, a highly methylated copia-like long terminal repeat retrotransposon insertion (4803 bp) in the promotor region of the R2 allele of BnSHP1.A9 repressed the expression of BnSHP1.A9, and thus contributed to pod shatter resistance. Finally, we showed a copia-like retrotransposon-based marker, BnSHP1.A9R2, can be used for marker-assisted breeding targeting the pod shatter resistance trait in oilseed rape.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
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