Drosophila GAGA factor directs histone H3.3 replacement that prevents the heterochromatin spreading
Drosophila GAGA factor directs histone H3.3 replacement that prevents the heterochromatin spreading
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DOI：
10.1101/gad.1503407
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发表时间：
2007-03-01
影响因子：
10.5
通讯作者：
Hirose, Susumu
中科院分区：
文献类型：
--
作者：
Nakayama, Takahiro;Nishioka, Kenichi;Hirose, Susumu
基因组表达状态的表观遗传维持对于发育至关重要。果蝇GAGA因子与FACT相互作用并调节染色质结构以维持基因表达。在这里，我们表明，GAGA因子- FACT复合物及其结合位点下游的白色基因是至关重要的位置效应杂色。有趣的是，在该位点，组蛋白H3 Lys 9甲基化出现下降，而H3 Lys 4甲基化出现峰值。GAGA因子和FACT通过HIRA在该位点的结合直接将组蛋白H3替换为H3.3，并在异染色质环境下维持白色表达。基于这些研究结果，我们提出GAGA因子和FACT-dependent替换赖氨酸9-甲基化组蛋白H3的H3.3抵消了沉默染色质的扩散。
Epigenetic maintenance of the expression state of the genome is critical for development. Drosophila GAGA factor interacts with FACT and modulates chromatin structure for the maintenance of gene expression. Here we show that the GAGA factor - FACT complex and its binding site just downstream from the white gene are crucial for position effect variegation. Interestingly there is a dip of histone H3 Lys 9 methylation and a peak of H3 Lys 4 methylation at this site. The GAGA factor and FACT direct replacement of histone H3 by H3.3 through association of HIRA at this site, and maintain white expression under the heterochromatin environment. Based on these findings we propose that the GAGA factor and FACT-dependent replacement of Lys 9-methylated histone H3 by H3.3 counteracts the spreading of silent chromatin.