Green Preparation Process, Characterization and Antitumor Effects of Doxorubicin-BSA-Dextran Nanoparticles
Green Preparation Process, Characterization and Antitumor Effects of Doxorubicin-BSA-Dextran Nanoparticles
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阿霉素-BSA-葡聚糖纳米粒的绿色制备工艺、表征及抗肿瘤作用
DOI：
10.1002/mabi.201000125
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发表时间：
2010-10-08
影响因子：
4.6
通讯作者：
Huang, Chong
中科院分区：
文献类型：
--
作者：
Deng, Wei;Li, Juan;Huang, Chong
本研究开发了一种简单、绿色的阿霉素-牛血清白蛋白-葡聚糖纳米粒的制备方法，并对阿霉素进行了高效包封。在Maillard反应产生的BSA-葡聚糖偶联物存在下，阿霉素与BSA的结合可以抑制未质子化阿霉素的自聚集。热处理后，BSA的凝胶化导致纳米颗粒的形成，并且阿霉素被固定在纳米颗粒内。葡聚糖壳使得纳米颗粒可分散在溶液中。纳米颗粒具有球形形态和约90 nm的流体动力学半径。重要的是，纳米颗粒可以显著延长小鼠腹水型肝癌H22荷瘤小鼠的生命。
In this report, we developed a simple and green process of simultaneous formation of doxorubicin-BSA-dextran nanoparticles in aqueous solution and high-effective encapsulation of doxorubicin. In the presence of BSA-dextran conjugates, which were produced by Maillard reaction, a binding of doxorubicin with BSA can suppress the self-aggregation of unprotonated doxorubicin. After a heat treatment, the gelation of BSA results in a formation of the nanoparticles and the doxorubicin was fixed inside the nanoparticles. The dextran shell makes the nanoparticles dispersible in solution. The nanoparticles have a spherical morphology and a hydrodynamic radius of about 90 nm. Importantly, the nanoparticles can significantly prolong the life of murine ascites hepatoma H22 tumor-bearing mice.