The extracellular proteome of Bacillus licheniformis grown in different media and under different nutrient starvation conditions
The extracellular proteome of Bacillus licheniformis grown in different media and under different nutrient starvation conditions
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DOI：
10.1002/pmic.200500091
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发表时间：
2006
期刊：
影响因子：
3.4
通讯作者：
B. Voigt;T. Schweder;M. Sibbald;D. Albrecht;A. Ehrenreich;J. Bernhardt;J. Feesche;K. Maurer;
中科院分区：
文献类型：
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作者：
B. Voigt;T. Schweder;M. Sibbald;D. Albrecht;A. Ehrenreich;J. Bernhardt;J. Feesche;K. Maurer;
现在完成的地衣芽孢杆菌DSM 13的基因组序列允许预测参与蛋白质分泌到细胞外环境中的基因以及预测易位的蛋白质。从序列中，预测296种蛋白质含有N末端信号肽，将其中大多数蛋白质引导至Sec系统，即革兰氏阳性细菌中的主要转运系统。采用双向电泳技术对B细胞外蛋白质组进行分析。研究了地衣在不同培养基中的生长情况。从凝胶上可见的约200个斑点中，鉴定出89个含有N末端信号序列或已知由Sec途径以外的其他机制分泌。B的胞外蛋白质组。地衣包括来自不同功能类别的蛋白质，如用于降解各种大分子的酶，参与细胞壁周转的蛋白质，鞭毛和噬菌体相关蛋白质以及一些功能未知的蛋白质。蛋白质分泌在静止生长期最高。此外，在复合培养基中生长的细胞比在基本培养基中生长的细胞分泌显著更高的蛋白质量。磷酸盐、碳和氮源的限制导致特定蛋白质的分泌，这些蛋白质可能参与对抗饥饿。
The now finished genome sequence of Bacillus licheniformis DSM 13 allows the prediction of the genes involved in protein secretion into the extracellular environment as well as the prediction of the proteins which are translocated. From the sequence 296 proteins were predicted to contain an N‐terminal signal peptide directing most of them to the Sec system, the main transport system in Gram‐positive bacteria. Using 2‐DE the extracellular proteome of B. licheniformis grown in different media was studied. From the approximately 200 spots visible on the gels, 89 were identified that either contain an N‐terminal signal sequence or are known to be secreted by other mechanisms than the Sec pathway. The extracellular proteome of B. licheniformis includes proteins from different functional classes, like enzymes for the degradation of various macromolecules, proteins involved in cell wall turnover, flagellum‐ and phage‐related proteins and some proteins of yet unknown function. Protein secretion is highest during stationary growth phase. Furthermore, cells grown in complex medium secrete considerably higher protein amounts than cells grown in minimal medium. Limitation of phosphate, carbon and nitrogen sources results in the secretion of specific proteins that may be involved in counteracting the starvation.