Comparison of the Sensititre YeastOne® Colorimetric Antifungal Panel with the Modified Clinical and Laboratory Standards Institute Broth Microdilution (M38-A) Method for Antifungal Susceptibility Testing of Dermatophytes
Comparison of the Sensititre YeastOne® Colorimetric Antifungal Panel with the Modified Clinical and Laboratory Standards Institute Broth Microdilution (M38-A) Method for Antifungal Susceptibility Testing of Dermatophytes
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Sensititre YeastOne® 比色抗真菌组合与用于皮肤癣菌抗真菌药敏测试的改良临床和实验室标准研究所肉汤微量稀释 (M38-A) 方法的比较
DOI：
10.1159/000158661
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发表时间：
2008
期刊：
影响因子：
3.3
通讯作者：
E. Martín
中科院分区：
文献类型：
--
作者：
Carmen Barroso Castro;M. Serrano;A. Valverde;J. Pemán;C. Almeida;E. Martín
背景：通过修改参考（微量稀释，CLSI M38-A）和比色法 Sensititre YeastOne® 比较不同皮肤癣菌种类对伊曲康唑（I）、氟康唑（F）和伏立康唑（V）的敏感性。微量稀释法对于临床实验室的常规药敏试验不太实用，因此需要使用其他方法。方法：我们研究了从临床标本中分离出的总共 46 株皮肤癣菌菌株。根据 CLSI M38-A 文件，使用 I、F 和 V 药物进行微量稀释参考药敏试验。 MIC 定义为 35°C 孵育 72 小时后产生 100%（I 和 V）和 50% 抑制（F）的最低药物浓度。通过颜色从粉红色变为蓝色或紫色来检测 Sensititre MIC。结果：两种方法（±2 个稀释度）对于须毛癣菌、红色毛癣菌和石膏小孢子菌的 I、F 和 V 的一致性水平分别为 30、53.3 和 83.3%、0、12.5 和 66.6%，以及 37.5、44.4 和 75%。通过微量稀释法测定 I、F 和 V 对须癣菌的 MIC50/90 (mg/l) 分别为 0.25/0.5、16/64 和 0.12/0.25，通过 Sensititre 法测定分别为 0.016/0.06、8/16 和 0.03/0.06。通过微量稀释法，红色毛癣菌的 I、F 和 V 的 MIC 分别为 0.25/1、8/64 和 0.25/0.5；通过 Sensititre 方法，I、F 和 V 的 MIC 分别为 0.008/0.03、2/8、0.016/0.03。对于石膏样霉，微量稀释法和 Sensititre 法的 MIC 分别为 0.5/1 (I)、/256 (F) 和 0.25/1 (V)，以及 0.016/0.25 (I)、16/256 (F) 和 0.06/0.25 (V)。结论：Sensititre 获得的 MIC 低于微量稀释法。两种方法之间的最佳相关性在须癣毛癣菌中的 V 中获得（>80%），但在红色毛癣菌中的相关性较低。尽管 Sensititre 方法在临床实验室中易于使用，但其与皮肤癣菌参考方法的一致性较差。
Background: To compare the susceptibility of different dermatophyte species to itraconazole (I), fluconazole (F) and voriconazole (V) by the modified reference (microdilution, CLSI M38-A) and the colorimetric method Sensititre YeastOne® . The microdilution method is not very practical for use in routine susceptibility testing in the clinical laboratory, thus necessitating the use of other methods. Methods: We studied a total of 46 dermatophyte strains isolated from clinical specimens. The microdilution reference susceptibility testing was performed following the CLSI M38-A document, using I, F and V drugs. The MIC were defined as the lowest drug concentration that produced 100% (I and V) and 50% inhibition (F) after 72 h incubation at 35°C. The Sensititre MIC were detected by a change in color from pink to blue or purple. Results: Agreement levels between the 2 methods (±2 dilutions) for I, F and V were 30, 53.3 and 83.3%, 0, 12.5 and 66.6% and 37.5, 44.4 and 75% for Trichophyton mentagrophytes, Trichophyton rubrum and Microsporumgypseum, respectively. The MIC50/90 (mg/l) of I, F and V for T. mentagrophytes were 0.25/0.5, 16/64 and 0.12/0.25 by the microdilution method and 0.016/0.06, 8/16 and 0.03/0.06 by the Sensititre method. The MIC for I, F and V for T. rubrum were 0.25/1, 8/64 and 0.25/0.5 by the microdilution and 0.008/0.03, 2/8, 0.016/0.03 by the Sensititre method. For M. gypseum, MIC were 0.5/1 (I), /256 (F) and 0.25/1 (V) as well as 0.016/0.25 (I), 16/256 (F) and 0.06/0.25 (V) by the microdilution and Sensititre methods, respectively. Conclusions: The MICs obtained were lower by the Sensititre than the microdilution method. The best correlation between both methods was obtained for V in T. mentagrophytes (>80%), but was low for T. rubrum. Although the Sensititre method is easy to use in a clinical laboratory, it shows poor agreement with the reference method for dermatophytes.