Trehalose in desiccated rotifers: a comparison between a bdelloid and a monogonont species
Trehalose in desiccated rotifers: a comparison between a bdelloid and a monogonont species
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DOI：
10.1016/j.cbpb.2004.10.019
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发表时间：
2004-12-01
影响因子：
2.3
通讯作者：
Santo, N
中科院分区：
文献类型：
--
作者：
Caprioli, M;Katholm, AK;Santo, N
为了应对干旱，蛭形轮虫和单殖体轮虫经历脱水生活，并被认为是合成保护性化学物质，这通常是糖。与大多数脱水生物相反，蛭形轮虫早期被证明缺乏作为保护性化学物质的海藻糖，更重要的是缺乏海藻糖合酶（tps）基因。它仍然有待评估，如果没有海藻糖是一个共同的特征，整个分类单元轮虫，或如果它是有限的蛭类，或特有的两个蛭类物种调查到目前为止。在这项研究中，脱水成蛭类物种（Macrotrachela quadricornifera）和休眠卵的单角体物种（褶皱臂尾轮虫）进行了分析，通过薄层色谱法和气相色谱法检测海藻糖的存在。蛭形轮虫中没有检测到海藻糖，而单角轮虫的脱水休眠卵中含有约占其干重0.35%的海藻糖。虽然很少，但B中海藻糖的存在。plicatilis表明在蛭形轮虫中不存在的海藻糖合酶基因存在于非蛭形轮虫中。(C)2004年爱思唯尔公司All rights reserved.
In response to drought bdelloid and monogonont rotifers undergo anhydrobiosis and are assumed to synthesize protective chemicals, which are commonly sugars. In contrast to most anhydrobionts, bdelloids have earlier been shown to lack trehalose as protective chemical, and more importantly to lack trehalose synthase (tps) genes. It remains to be assessed if the absence of trehalose is a characteristic common to the entire taxon Rotifera, or if it is limited to bdelloids, or is peculiar to the two bdelloid species investigated so far. In this study, anhydrobiotic adults of a bdelloid species (Macrotrachela quadricornifera) and resting eggs of a monogonont species (Brachionus plicatilis) were analysed by thin layer chromatography and gas chromatography to detect the presence of trehalose. No trehalose was detected in the bdelloid, while the anhydrobiotic resting egg of the monogonont rotifer contained about 0.35% trehalose of its dry weight. Although very little, the presence of trehalose in B. plicatilis suggests that the trehalose synthase genes, absent in bdelloid rotifers, are present in non-bdelloid rotifers. (C) 2004 Elsevier Inc. All rights reserved.