CYTOPLASMIC PH AND THE REGULATION OF THE DICTYOSTELIUM CELL-CYCLE
CYTOPLASMIC PH AND THE REGULATION OF THE DICTYOSTELIUM CELL-CYCLE
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DOI：
10.1016/0092-8674(85)90237-5
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发表时间：
1985-12-01
期刊：
影响因子：
64.5
通讯作者：
MOOLENAAR, WH
中科院分区：
文献类型：
--
作者：
AERTS, RJ;DURSTON, AJ;MOOLENAAR, WH
用pH“零点法”测定了盘基网柄藻同步种群细胞周期的细胞质pH(PH)。有一个phi周期与DNA复制周期密切对应，间期的最低pH为7.20，S期和有丝分裂的pH峰值为7.45。当DNA复制被羟基脲阻断时，phi继续振荡，周期与不受抑制的分裂周期相似。即使当蛋白质合成被环己亚胺抑制时，周期性的phi循环仍然存在。通过apprx在指数级生长的细胞中人工提高phi。0.1个单位会导致蛋白质和DNA合成速度增加几倍。我们的结论是，Dictyostelius细胞中存在一个独立于蛋白质和DNA合成的自主Phi振荡器，它可能在细胞周期的计时和调节中发挥重要作用。
Cytoplasmic pH (pHi) was monitored during the cell cycle of synchronous populations of Dictyostelium discoideum by means of a pH "null point" method. There is a cycle of pHi that closely corresponds to the DNA replication cycle, with a minimum of pH 7.20 in interphase and a peak of pH 7.45 during S phase and mitosis. When DNA replication is blocked by hydroxyurea, pHi continues to oscillate with a similar period as the uninhibited division cycle. Even when protein synthesis is inhibited by cycloheximide the periodic pHi cycles persist. Artificially raising pHi in exponentially growing cells by .apprx. 0.1 units causes a severalfold increase in the rates of protein and DNA synthesis. We conclude that an autonomous pHi oscillator exists in Dictyostelium cells, which operates independently of protein and DNA synthesis and which may have a major role in the timing and regulation of the cell cycle.