Plasmid gene organization: naphthalene/salicylate oxidation.
Plasmid gene organization: naphthalene/salicylate oxidation.
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质粒基因组织：萘/水杨酸氧化。
DOI：
10.1073/pnas.79.3.874
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发表时间：
1982
影响因子：
11.1
通讯作者：
I. C. Gunsalus
中科院分区：
文献类型：
--
作者：
K. Yen;I. C. Gunsalus
萘代谢的基因位于nah 7上，一个83 kb的质粒，在水杨酸盐控制下的两个基因簇中。通过插入转座子Tn 5形成的极性突变允许检测由约7-kb调控基因区分隔的两个约10-kb DNA片段内的转录方向和基因组织。指定萘转化为水杨酸的基因簇位于EcoRI释放的25-kb DNA片段A的左侧起始处附近;通过邻苯二酚易位的水杨酸途径的基因簇位于右侧末端附近，延伸到相邻的5.9-kb片段C。遗传组织和调节类似于恶臭假单胞菌MT 2中甲苯/二甲苯氧化的tol质粒编码的“上”和“下”途径。
Genes for naphthalene metabolism are localized on nah7, an 83-kilobase (kb) plasmid, in two gene clusters under salicylate control. Polar mutations formed by insertion of the transposon Tn5 permit detection of the transcription direction and the gene organization within two approximately 10-kb DNA segments separated by a approximately 7-kb regulatory gene region. The gene cluster specifying conversion of naphthalene to salicylate lies near the left initiation of a 25-kb DNA fragment A released by EcoRI; that for the salicylate pathway via catechol meta-fission lies near the right terminus with extension into the adjoining 5.9-kb fragment C. The genetic organization and regulation resemble the tol plasmid-encoded "upper" and "lower" pathways of toluene/xylene oxidation in Pseudomonas putida mt2.