PHOSPHOLIPID UPTAKE BY PLASMODIUM-KNOWLESI INFECTED ERYTHROCYTES
PHOSPHOLIPID UPTAKE BY PLASMODIUM-KNOWLESI INFECTED ERYTHROCYTES
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DOI：
10.1016/0014-5793(88)80765-8
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发表时间：
1988-05-23
期刊：
影响因子：
3.5
通讯作者：
VANDEENEN, LLM
中科院分区：
文献类型：
--
作者：
MOLL, GN;VIAL, HJ;VANDEENEN, LLM
本文研究了诺氏疟原虫感染红细胞对磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和磷脂酰丝氨酸（PS）的摄取。而摄取的磷脂，在磷脂转移蛋白的情况下，是可以忽略不计的控制细胞，感染的细胞可以纳入相当数量的添加磷脂。脂质转移蛋白的存在增强了摄取。双标记[3H]油酸酯，[14 C]胆碱）PC在摄取后未发生任何明显的重塑，这强烈表明血浆PC本身用于寄生虫膜的生物发生。通过观察这些脂质分别转化为PE和PC，证实了细胞外提供的PS和PE向红细胞内寄生虫的运输和寄生虫对这些脂质的利用。这些转化的程度和速率取决于磷脂被引入感染细胞的方式。
The uptake of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) inPlasmodium knowlesiinfected erythrocytes has been studied. Whereas uptake of phospholipids, in the absence of phospholipid transfer proteins, is negligible in control cells, the infected cells can incorporate considerable amounts of added phospholipids. The uptake is enhanced by the presence of lipid transfer proteins. Doubly labeled [3H]oleate, [14C]choline) PC does not undergo any appreciable remodelling following uptake, which strongly suggests that plasma PC is used as such for the biogenesis of the parasite membranes. Transport of extracellularly offered PS and PE towards the intraerythrocytic parasite and utilization of these lipids by the parasite are confirmed by the observation that these lipids are converted into respectively PE and PC. The extent and rate of these conversions depend on the way the phospholipids are introduced into the infected cells.