Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl.
Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl.
复制标题
墨西哥蝾螈发育过程中肌原纤维诱导 RNA 在心脏 TnT 表达中的作用。
DOI：
10.1016/j.bbrc.2007.03.064
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发表时间：
2007
影响因子：
3.1
通讯作者：
Lemanski,La
中科院分区：
文献类型：
--
作者：
Sferrazza,GianFranco;Zhang,Chi;Jia,Pingping;Lemanski,SharonL;Athauda,Gagani;Stassi,Alyssa;Halager,Kristine;Maier,JenniferA;Rueda-de-Leon,Elena;Gupta,Amit;Dube,Syamalima;Huang,Xupei;Prentice,HowardM;Dube,DipakK;Lemanski,La
The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene ``c”, for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts. Myofibril-inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac troponin T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart.
