Emergence of clinical isolates of Pseudomonas asiatica and Pseudomonas monteilii from Japan harbouring an acquired gene encoding a carbapenemase VIM-2
Emergence of clinical isolates of Pseudomonas asiatica and Pseudomonas monteilii from Japan harbouring an acquired gene encoding a carbapenemase VIM-2
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DOI：
10.1099/jmm.0.001258
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发表时间：
2021-01-01
影响因子：
3
通讯作者：
Fujita, Jiro
中科院分区：
文献类型：
--
作者：
Tohya, Mari;Uechi, Kohei;Fujita, Jiro
亚洲假单胞菌和蒙氏假单胞菌属于恶臭假单胞菌系统发育组，偶尔从临床样本中分离出来，部分原因是它们经常在临床实验室中被误认为恶臭假单胞菌。有5份报告描述了这些种属的碳青霉烯类耐药临床分离株。从粪便样品中分离出亚洲巴斯德菌和孟氏巴斯德菌的碳青霉烯类耐药菌株。使用Illumina MiSeq对这些分离株进行测序，并基于使用OrthoANI算法对其全基因组序列进行比较，使用平均核苷酸同一性（ANI）重新鉴定。通过脉冲场凝胶电泳（PFGE）评估分离株的克隆相关性。通过Southern印迹检测携带bla（Vim-2）的质粒的大小。从日本一家医院的5名患者的粪便样本中获得了6株碳青霉烯类耐药临床分离株亚洲青霉菌（2株分离株）和孟氏青霉菌（4株分离株）。所有分离株均携带bla（Vim-2）。在PFGE分析中，两个亚洲假单胞菌分离物具有不同的模式，两者都具有23 kb质粒。在PFGE分析中具有相似模式的4个蒙氏毕赤酵母分离株中，3个具有320 kb质粒，1个具有240 kb质粒。320/240 kb和23 kb质粒的遗传环境不同。结果有力地表明，碳青霉烯类耐药的亚洲假单胞菌和产生金属β-内酰胺酶的蒙氏假单胞菌正在日本出现。这是首次在日本报道碳青霉烯类耐药的亚洲青霉和孟氏青霉。
Pseudomonas asiatica and Pseudomonas monteilii, belonging to the Pseudomonas putida phylogenetic group, are occasionally isolated from clinical samples, partly because they are often misidentified as P. putida in clinical laboratories. There are five reports describing carbapenem-resistant clinical isolates of these species. Carbapenem-resistant strains of P. asiatica and P. monteilii were isolated from stool samples. These isolates were sequenced using Illumina MiSeq and reidentified using average nucleotide identity (ANI) based on comparisons of their whole-genome sequences using the OrthoANI algorithm. The clonal relatedness of the isolates was assessed by pulse-field gel electrophoresis (PFGE). The size of plasmids conveying bla(VIM-2) was examined by Southern blotting. A total of six carbapenem-resistant clinical isolates of P. asiatica (two isolates) and P. monteilii (four isolates) were obtained from stool samples from five patients in a Japanese hospital. All isolates harboured bla(VIM-2). The two isolates of P. asiatica had a different pattern in the PFGE analysis, with both having a 23 kb plasmid. Of the four isolates of P. monteilii with similar patterns in the PFGE analysis, three had 320 kb plasmids and one had a 240 kb plasmid. The genetic environments of the 320/240 kb and 23 kb plasmids differed. The results strongly indicated that carbapenem-resistant P. asiatica and P. monteilii producing metallo-beta-lactamase are emerging in Japan. This is the first report of carbapenem-resistant P. asiatica and P. monteilii in Japan.