Experimental test of microbiome protection across pathogen doses reveals importance of resident microbiome composition
Experimental test of microbiome protection across pathogen doses reveals importance of resident microbiome composition
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DOI：
10.1093/femsec/fiab141
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发表时间：
2021-10-09
影响因子：
4.2
通讯作者：
Hawley，Dana M.
中科院分区：
文献类型：
--
作者：
Weitzman，Chava L.;Rostama，Bahman;Hawley，Dana M.
寄生在宿主组织中的微生物可以与入侵的病原体和宿主生理学相互作用，从而改变病原体的生长和疾病的表现。先前对家雀（墨西哥血雀）的研究发现，常驻眼部微生物组对结膜感染和由相对高剂量的鸡毒支原体引起的疾病具有保护作用。在这里，我们使用野生捕获的家雀来实验性地检查常驻眼部微生物组的保护作用是否随入侵病原体的剂量而变化。我们假设在低M时，骨骼保护最强。鸡败血症接种剂量，因为常驻微生物组将较少被入侵的病原体破坏。我们的五M。鸡败血症剂量处理与抗生素处理在M前干扰常驻微生物是完全因子。鸡败血症接种出乎意料的是，我们没有发现在任何病原体接种剂量下驻留微生物组的保护作用的迹象，这与先前的工作不一致。在我们的实验开始时，眼部细菌群落与先前在当地野生捕获的家雀中报道的显着不同，可能导致这种差异。这些可变的结果强调，自然系统中基于微生物组的保护可能是环境依赖性的，群落组成的自然变化可能会改变自由生活动物中常驻微生物组的功能。
The commensal microbes inhabiting a host tissue can interact with invading pathogens and host physiology in ways that alter pathogen growth and disease manifestation. Prior work in house finches (Haemorhous mexicanus) found that resident ocular microbiomes were protective against conjunctival infection and disease caused by a relatively high dose ofMycoplasma gallisepticum. Here, we used wild-caught house finches to experimentally examine whether protective effects of the resident ocular microbiome vary with the dose of invading pathogen. We hypothesized that commensal protection would be strongest at lowM. gallisepticuminoculation doses because the resident microbiome would be less disrupted by invading pathogen. Our fiveM. gallisepticumdose treatments were fully factorial with an antibiotic treatment to perturb resident microbes just prior toM. gallisepticuminoculation. Unexpectedly, we found no indication of protective effects of the resident microbiome at any pathogen inoculation dose, which was inconsistent with the prior work. The ocular bacterial communities at the beginning of our experiment differed significantly from those previously reported in local wild-caught house finches, likely causing this discrepancy. These variable results underscore that microbiome-based protection in natural systems can be context dependent, and natural variation in community composition may alter the function of resident microbiomes in free-living animals.