Strategies for Oral Mucosal Repair by Engineering 3D Tissues with Pluripotent Stem Cells
Strategies for Oral Mucosal Repair by Engineering 3D Tissues with Pluripotent Stem Cells
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DOI：
10.1089/wound.2013.0480
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发表时间：
2014-12-01
影响因子：
4.9
通讯作者：
Garlick, Jonathan A.
中科院分区：
文献类型：
--
作者：
Hewitt, Kyle J.;Shamis, Yulia;Garlick, Jonathan A.
重要性：人诱导多能干细胞（iPSC）可以分化为具有广泛细胞表型的患者特异性细胞，并提供用于治疗用途的自体细胞的替代来源。最近的研究表明，iPSC衍生的成纤维细胞显示出增强的细胞功能，这表明iPSC最终可能成为再生疗法的干细胞的重要来源。最新进展：发现将体细胞重编程为多能细胞的方法为它们在个性化再生疗法中的应用开辟了令人兴奋的途径。来自iPSC的功能性细胞类型的受控分化提供了成纤维细胞的补充来源。有有趣的证据表明，iPSC重编程和随后分化为成纤维细胞谱系可以改善细胞功能特性。增强iPSC衍生的成纤维细胞的生物学效能可能使开发新的，个性化的干细胞疗法来治疗口腔diseases.Critical Issues：在iPSC衍生的细胞可以用作实际的口腔疗法之前，需要解决许多问题。这将包括理解为什么iPSC衍生的细胞倾向于沿着与其细胞来源相关的谱系分化途径，筛选iPSC衍生的细胞以确保其安全性和表型稳定性，并开发工程化的三维组织模型以优化其功能和功效，用于未来的治疗性移植。未来的研究将需要解决如何开发有效的方法来将iPSC衍生的成纤维细胞递送和整合到口腔粘膜中。这将需要更好地了解如何利用它们的生物学潜力进行专门针对口腔粘膜的再生治疗。
Significance: Human-induced pluripotent stem cells (iPSC) can be differentiated into patient-specific cells with a wide spectrum of cellular phenotypes and offer an alternative source of autologous cells for therapeutic use. Recent studies have shown that iPSC-derived fibroblasts display enhanced cellular functions suggesting that iPSC may eventually become an important source of stem cells for regenerative therapies.Recent Advances: The discovery of approaches to reprogram somatic cells into pluripotent cells opens exciting avenues for their use in personalized, regenerative therapies. The controlled differentiation of functional cell types from iPSC provides a replenishing source of fibroblasts. There is intriguing evidence that iPSC reprogramming and subsequent differentiation to fibroblast lineages may improve cellular functional properties. Augmenting the biological potency of iPSC-derived fibroblasts may enable the development of novel, personalized stem cell therapies to treat oral disease.Critical Issues: Numerous questions need to be addressed before iPSC-derived cells can be used as a practical oral therapy. This will include understanding why iPSC-derived cells are predisposed towards differentiation pathways along lineages related to their cell of origin, screening iPSC-derived cells to ensure their safety and phenotypic stability and developing engineered, three-dimensional tissue models to optimize their function and efficacy for future therapeutic transplantation.Future Directions: Future research will need to address how to develop efficient methods to deliver and integrate iPSC-derived fibroblasts into the oral mucosa. This will require an improved understanding of how to harness their biological potency for regenerative therapies that are specifically targeted to the oral mucosa.