A dual-site simultaneous binding mode in the interaction between parallel-stranded G-quadruplex [d(TGGGGT)]4 and cyanine dye 2,2'-diethyl-9-methyl-selenacarbocyanine bromide.
A dual-site simultaneous binding mode in the interaction between parallel-stranded G-quadruplex [d(TGGGGT)]4 and cyanine dye 2,2'-diethyl-9-methyl-selenacarbocyanine bromide.
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DOI：
10.1093/nar/gks1328
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发表时间：
2013-02-01
影响因子：
14.9
通讯作者：
Tang Y
中科院分区：
文献类型：
--
作者：
Gai W;Yang Q;Xiang J;Jiang W;Li Q;Sun H;Guan A;Shang Q;Zhang H;Tang Y
近年来，G-四链体作为潜在的癌症相关治疗和检测靶点引起了越来越多的关注。为了检测目的，高特异性是G-四联体探针设计中需要考虑的最重要因素之一。众所周知，末端堆积和凹槽结合是两种主要的四联体配体结合模式，目前大多数报道的G-四联体探针都是基于前者设计的，已被证明在四联体和非四联体之间表现出良好的选择性。由于G-四联体的凹槽也具有一些独特的化学性质，因此可以推断，能够同时与某些G-四联体的凹槽和G-四分体位点相互作用的探针在区分不同四联体方面可能具有更高的特异性。在本文中，我们报道了一种花青染料作为一种潜在的新型探针支架，它可以同时占据 5' 端外部 G-四联体和 G-四联体的相应凹槽。通过使用各种光谱和核磁共振技术，我们给出了这种双位点同时结合模式的详细结合表征。初步结果表明，这种模式可能为平行链 G 四链体提供高度特异性的识别。这些发现和结构解析可能为开发高度特异性的 G 四链体探针提供一些线索。
G-quadruplexes have attracted growing attention as a potential cancer-associated target for both treatment and detection in recent years. For detection purpose, high specificity is one of the most important factors to be considered in G-quadruplex probe design. It is well known that end stacking and groove binding are two dominated quadruplex-ligand binding modes, and currently most reported G-quadruplex probes are designed based on the former, which has been proven to show good selectivity between quadruplexes and non-quadruplexes. Because groove of G-quadruplex also has some unique chemical properties, it could be inferred that probes that can interact with both the groove and G-tetrad site of certain G-quadruplexes simultaneously might possess higher specificity in aspects of discriminating different quadruplexes. In this article, we report a cyanine dye as a potential novel probe scaffold that could occupy both the 5′-end external G-tetrad and the corresponding groove of the G-quadruplex simultaneously. By using various spectrum and nuclear magnetic resonance techniques, we give a detailed binding characterization for this dual-site simultaneous binding mode. A preliminary result suggests that this mode might provide highly specific recognition to a parallel-stranded G-quadruplex. These findings and the structural elucidation might give some clues in aspects of developing highly specific G-quadruplex probes.
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G-四链体配体与人染色体末端的优先结合。
影响因子：
14.9
作者：
Granotier, C;Pennarun, G;Riou, L;Hoffschir, F;Gauthier, LR;De Cian, A;Gomez, D;Mandine, E;Riou, JF;Mergny, JL;Mailliet, P;Dutrillaux, B;Boussin, FD
通讯作者：
Boussin, FD
影响因子：
15
作者：
Brooker, LGS;White, FL
通讯作者：
White, FL
影响因子：
--
作者：
BERGMANN, K;OKONSKI, CT
通讯作者：
OKONSKI, CT
影响因子：
15
作者：
Han, FXG;Wheelhouse, RT;Hurley, LH
通讯作者：
Hurley, LH
影响因子：
2.9
作者：
HOOVER, WG
通讯作者：
HOOVER, WG