Cytochemical localization of glucose-6-phosphatase activity in the central nervous system of the rat
Cytochemical localization of glucose-6-phosphatase activity in the central nervous system of the rat
复制标题
大鼠中枢神经系统葡萄糖-6-磷酸酶活性的细胞化学定位
DOI：
10.1016/0006-8993(76)90011-1
复制
发表时间：
1976
期刊：
影响因子：
2.9
通讯作者：
E. Sandborn
中科院分区：
文献类型：
--
作者：
H. Stephens;E. Sandborn
在电镜水平上证实了CNS神经元中葡萄糖-6-磷酸酶（G-6-Pase）的组织化学检测。葡萄糖-6-磷酸（G-6-P）和α-甘油磷酸酯（α-gP）都可以作为底物来定位该酶的反应产物，我们在大脑皮层、小脑和脑干的所有细胞类型中都发现了这种酶。这种反应在大的神经元中最为突出，如小脑的浦肯野细胞和大脑皮层的锥体细胞。这是由于它们含有大量粗糙和光滑的内质网，这是G-6-Pase活性的超微结构部位。可以定量测量脑匀浆和微粒体组分中G-6-P和α-gP的水解，这是细胞化学可证明活性的生化相关。这些结果要求对先前的生化证据进行重新评估，这些证据否定了脑G-6-Pase的存在，因此对当前有关脑葡萄糖代谢调节的概念进行重新评估。
The histochemical detection of glucose-6-phosphatase (G-6-Pase) in neurons of the CNS has been confirmed at the level of the electron microscope. Both glucose-6-phosphate (G-6-P) and α-glycerophosphate (α-gP) can be used as substrates to localize the reaction product of this enzyme, which we have found in all cell types of the cerebral cortex, cerebellum and brain stem. The reaction was most prominent in large neurons, such as the Purkinje cells of the cerebellum and the pyramidal cells of the cerebral cortex. This is due to their extensive content of rough and smooth endoplasmic reticulum, the ultrastructural sites of G-6-Pase activity. It was possible to measure quantitatively the hydrolysis of G-6-P and α-gP in brain homogenates and also in microsomal fractions, the biochemical correlate of the cytochemically demonstrable activity. These results call for a reappraisal of the previous biochemical evidence, which negates the existence of brain G-6-Pase, and consequently a reassessment of current concepts pertaining to the metabolic regulation of brain glucose.