Protein phosphatase type 2C PP2CA together with ABI1 inhibits SnRK2.4 activity and regulates plant responses to salinity.
Protein phosphatase type 2C PP2CA together with ABI1 inhibits SnRK2.4 activity and regulates plant responses to salinity.
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DOI：
10.1080/15592324.2016.1253647
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发表时间：
2016-12
影响因子：
2.9
通讯作者：
Dobrowolska G
中科院分区：
文献类型：
--
作者：
Krzywińska E;Kulik A;Bucholc M;Fernandez MA;Rodriguez PL;Dobrowolska G
蛋白磷酸酶 2C (PP2C) 是植物对非生物胁迫反应的重要调节因子。已确定进化枝 A PP2C 抑制 ABA 激活的 SNF1 相关蛋白激酶 2 (SnRK2)。我们最近发表的结果表明，ABI1（PP2C 进化枝 A 的成员）也是 SnRK2.4 的负调节因子，SnRK2.4 是一种不响应 ABA 激活的激酶。在这里，我们表明该进化枝的另一个成员 - PP2CA，与 SnRK2.4 相互作用并抑制 SnRK2.4。 abi1-2 pp2ca-1 突变体中盐诱导的 SnRK2.4/SnRK2.10 活性高于野生型或单个 abi1 或 pp2ca 突变体，表明两种磷酸酶都是 SnRK2.4 的抑制剂，并且至少部分冗余。此外，PP2CA 与 ABI1 和 SnRK2.4 一起调节根系生长以响应盐度。
Protein phosphatases 2C (PP2Cs) are important regulators of plant responses to abiotic stress. It is established that clade A PP2Cs inhibit ABA-activated SNF1-related protein kinases 2 (SnRK2s). Our recently published results show that ABI1, a member of clade A of PP2C is also a negative regulator of SnRK2.4, a kinase not activated in response to ABA. Here, we show that another member of this clade - PP2CA, interacts with and inhibits SnRK2.4. The salt-induced SnRK2.4/SnRK2.10 activity is higher in abi1–2 pp2ca-1 mutant than in wild type or single abi1 or pp2ca mutants, indicating that both phosphatases are inhibitors of SnRK2.4 and are at least partially redundant. Moreover, PP2CA together with ABI1 and SnRK2.4 regulates root growth in response to salinity.